Native and recombined lipoproteins have been studied by (31)P nuclear magnetic resonance spectroscopy. Very low-, low-, and high-density lipoproteins exhibited characteristic spectra. The main resonances were assigned to phosphatidylcholine and sphingomyelin. Relaxation times for these phospholipids were separately measured in low-density lipoproteins and high-density lipoproteins. The effect o...

High density lipoproteins of rat blood plasma were labeled in vitro with radioiodinated apolipoprotein E and biologically with [3H]cholesteryl esters. These two components, present in high density lipoproteins separated from serum of normal or cholesterol-fed rats by molecular sieve chromatography, were removed slowly from perfused livers and the labeled apolipoprotein E was also removed slowly...

objectives in this study the association of phenotypes and activity of butyrylcholinesterase (buche) with serum level of lipid-lipoprotein and apolipoproteins during various phases of menstrual-cycle was determined. methods the study population consisted of 22 healthy women aged 19–25 years with regular menstrual cycles, 26–30 days in length. the serum levels of lipids, apolipoproteins, and buc...

We describe an ultracentrifugation method for isolating the different lipoprotein classes relatively quickly. In this method the very-low-density lipoproteins are first separated by non-density-adjusted ultracentrifugation. The resulting infranatant material is then stained with Coomassie Brilliant Blue R-250 and ultracentrifuged in a density gradient. The intermediate-density lipoproteins (IDL...

We have developed a procedure for labeling lipoproteins with the fluorescent probe 3,3'-dioctadecylindocarbocyanine (Dil) and have used Dil-labeled native and acetoacetylated lipoproteins to differentiate macrophages from fibroblasts in mixed cell culture. Lipoproteins labeled with this probe were suitable for the direct viewing of their binding and internalization by cells in vitro. The labeli...

Rat livers were perfused for 6 h without added plasma proteins using washed erythrocytes and buffer in a recirculating system. An inhibitor to the enzyme lecithin-cholesterol acyltransferase (5,5'-dithionitrobenzoic acid) was added in some experiments to prevent modification of substrate-lipids contained in secreted lipoproteins. The inhibitor did not detectably alter hepatic ultrastructure or ...

The fractionation of lipoproteins of normal and hyperlipidemic subjects on a column of 2% agarose was compared with ultracentrifugation and paper electrophoresis procedures. The following results were obtained. (a) Plasma lipoproteins were eluted successively from the column in the four overlapping peaks of chylomicrons, very low density lipoproteins, low density lipoproteins, and high density ...

to evaluate the correlations of the serum concentrations of trace elements with lipids and lipoproteins in water buffalo (bubalus bubalis), the serum concentrations of cholesterol, triglyceride, total lipids, very low density lipoproteins (vldl-cholesterol), low density lipoproteins (ldl-cholesterol) and high density lipoproteins (hdl-cholesterol) and their correlations with serum concentration...

Causative factors of uremia-associated atherosclerosis are complex. However, it is likely that atherogenic lipoproteins accumulated in plasma are involved. Remnant lipoproteins are atherogenic and are frequently observed in uremic plasma. LDL from uremic patients has been shown to be susceptible to in vitro peroxidation, suggesting that oxidized LDL (ox-LDL) could be excessively generated in th...

To evaluate the concentrations of serum lipids and lipoproteins and their correlations together and withthyroid hormones in gray necked ostrich (Struthio camelus), the serum levels of cholesterol, triglyceride,total lipids, very low density lipoproteins (VLDL-cholesterol), low density lipoproteins (LDL-cholesterol)and high density lipoproteins (HDL-cholesterol) and their correlations with tri-i...