objective(s) a sensitive liquid chromatographic method for the analysis of clarithromycin- a macrolide antibiotic- in human serum, using pre-column derivatization with 9-fluorenylmethyl chloroformate (fmoc-cl) is described. materials and methods the method involved liquid-liquid extraction of the drug and an internal standard (amantadine) followed by pre-column derivatization of the analytes wi...

urinary 1-hydroxypuren (1-ohp) is commonly used as a major metabolite and biological indicator of the overall exposure to polycyclic aromatic hydrocarbons (pahs). for evaluation of human exposure to such compounds, biological monitoring is an essential process, in which, preparation of samples is one of the most time-consuming and error-prone aspects prior to chromatographic techniques. in this...

Background & Aims: In this study, using a liquid-liquid microextraction method for pre-concentration trace amounts of aflatoxins, the amount of Aflatoxins B1 and B2 in powdered milk was determined. Determination of aflatoxins was done by using high-performance liquid chromatography coupled with fluorescent detector. Method: Samples were extracted by immunoaffinity column (IAC) clean-up, and the...

this research includes both experimental and cfd investigation in the hydrodynamic behavior of a new type of column tray introduced as “concap” tray. the proposed column tray is used in contactor columns especially in stripping columns. the hydrodynamics of this concap tray is investigated in a 1.2m in diameter column. using air-water system, the experiments were performed for different liquid ...

A standard mixture of 25 short-chain fatty acids was resolved by high-performance liquid chromatography, using an Aminex HPX-87 column. The acids produced in culture media by anaerobic bacteria were analyzed by high-performance liquid chromatography after extraction with ether and reextraction into a small volume of 0.1 N NaOH. The presence of fumaric acid in culture extracts of Peptostreptococ...

high-performance liquid chromatography (hplc) methods employing ultraviolet (uv) detector are not sufficiently sensitive to measure the low plasma concentrations following single oral dose of imipramine. therefore, in the present study a simple, rapid and yet sensitive hplc method with uv detection was developed and validated for quantitation of imipramine in human plasma samples. an efficient ...

the aim of this study was to develop an easy analytical method for determining flam prop-m- iso propyl residues in honey by high performance liquid chromatography with uv detection (hplc/uv) for routine analysis. chromatographic separation was carried out at room temperature on a zorbax eclipse xdb- c18, 100 å pore size, 5 μm particle size (300 mm × 3 mm i.d.) column using a mobile phase of met...

OBJECTIVE The stable quality of Chinese herbal medicines is a critical factor for their reliable clinical efficiency. An improved liquid-liquid extraction procedure and a liquid chromatographic method were developed to simultaneously analyze five anthraquinones (aloe-emodin, rhein, emodin, chrysophanol and physcion) in a Chinese traditional hospital preparation, Fuyankang mixture, in order to q...

An isocratic high-performance liquid chromatographic method for the determination of testosterone (T) and epitestosterone (ET) in human urine using liquid-liquid or solid-phase extraction (SPE) is developed and validated. The optimum separation is achieved using a Hypersil C(18) column, water-acetonitrile (57:43, v/v) as the mobile phase and UV-absorbance detection at 245 nm. The recoveries obt...

Process engineers can be faced with the task of understanding and optimizing the performance of an existing liquid-liquid extraction (extraction) column. Often times, an extraction column has been operating many years, and the performance is not well understood by the personnel responsible for its operation. Since this technology is not treated comprehensively during most under-graduate Chemica...