We have prepared murine monoclonal antibodies against isolated human bone alkaline phosphatase (ALP, EC 3.1.3.1). Hybridoma supernates were separately screened for reactivity against both human liver and bone ALP. Although most antibody-positive hybrids showed similar reactivity against both isoenzymes, one hybridoma produced an antibody that interacted preferentially with liver ALP. This antib...

We have determined the effect of diabetes mellitus and starvation/re-feeding on serum alkaline phosphatase (ALP) isoenzymes in the rat. Both ketotic (DKA) and nonketotic (NKDM) diabetic rats showed elevated total ALP activity, primarily due to an increase in intestinal and bone/liver ALP isoenzyme in the NKDM and DKA groups, respectively. There was a decrease in intestinal ALP activity in 3 day...

Human placenta alkaline phosphatase (HP-ALP), a glycoprotein, was stained histochemically for the purpose of examining the concanavalin A (Con A) binding sites on the cell surface. HP-ALP was bound to the cell surface by Con A. This simple method successfully detected Con A binding sites on the cell surface.

INTRODUCTION Echinococcus granulosus infection may be transmitted between humans and animals. Hydatid disease refers to an infection with the parasite larva in humans, and echinococcosis is restricted to infection with the adult stage in carnivorous animals [1]. Alkaline phosphatase (ALP) plays an important role in metabolism, physiology, immunology, and nutrients absorption of the cystic echin...

The aim of the present study was to investigate effect antihistamines(Chlorpheneramineand Fexofenadine) on (alkaline phosphatase, aspartate amino transpherase and alanine transferase) in tissue fertilized hens eggs embryos as indicator damage. injection hen's with antihistamines Fexofenadine at 12th day incubation doses (0,5,0,1mg/ egginto air sell)after 24 hour treatment induce significantly d...

STATEMENT OF THE PROBLEM The present constituents of gingival crevicular fluid (GCF) can reflect the changes occurring in underlying tissues. Considering variety of biologic bone markers, alkaline phosphatase and acid phosphatase have been examined as bone turn over markers in orthodontic tooth movement. PURPOSE The current study designed in a longitudinal pattern to determine the changes of ...

A one-step, single use, disposable Alkaline Phosphatase (ALP) biosensor has been developed. It is based on the detection of phenol produced by an ALP enzymatic reaction. It can operate at 25 °C in a pH 10 medium. It measures ALP of 0-300 IU/L. The permissible concentrations of glucose, ascorbic acid and urea without interference are 10 mM/L, 5 mg/L and 400 mg/L, respectively. Experimental resul...

Alkaline phosphatase (ALP) flare phenomenon documented as scintigraphic flare phenomenon due to elevated serum ALP levels produced by osteoblasts reflects an osteoblastic reaction in response to the effective therapy of non-small cell lung cancer (NSCLC). Here, we report a case of ALP flare following gefitinib treatment for NSCLC. We also retrospectively analyzed the prevalence of ALP flare in ...

Reaching the differential diagnosis of tuberculosis and sarcoidosis can be difficult due to granulomatous inflammation. Determining alkaline phosphatase (ALP) activity in serum for sarcoidosis. This study comprised 104 acid-fast bacilli (AFB) positive patients with pulmonary tuberculosis, 90 biopsy-proven 43 healthy controls were included. ALP was measured at first admission patients. There a s...

The aim of this work was to assess the relationship of both total alkaline phosphatase (ALP) and bone-specific alkaline phosphatase (BsALP) with the course and outcome of operatively treated long-bone fractures. The activity of total ALP and BsALP was measured in 41 patients with a long bone fracture, comprising 26 men and 15 women. All patients were treated operatively. Total ALP and BsALP lev...