Background Single-use bioreactors continue to gain large interest in the biopharmaceutical industry. They are excessively used for mammalian cell cultivations, e.g. production of monoclonal antibodies and vaccines [1]. This is motivated by several advantages of these bioreactors like reduced risk of cross contaminations or shortening lead times [2]. Singleuse bioreactors differ in terms of shap...

Continuous bioreactors are critical unit operations in a wide variety of biotechnological processes. While they can be viewed as chemical reactors, bioreactors offer unique modeling and control challenges due to the complexity of the underlying biochemical reactions and the distributed properties of the cell population. The dynamic behavior of continuous bioreactors can be strongly affected by ...

Rationale and significance. Recombinant therapeutic proteins are frequently produced in mammalian cells cultivated in suspension in large-scale stirred tank bioreactors. There are three major drawbacks to stirred-tank bioreactors. First, they are expensive to purchase and maintain, limiting their availability to some potential users. Second, they have not been adapted to volumetric scales less ...

Experimental bioreactors operated as recirculated closed systems were inoculated with bacterial cultures that utilized methane, propane, and tryptone-yeast extract as aerobic carbon and energy sources and degraded trichloroethylene (TCE). Up to 95% removal of TCE was observed after 5 days of incubation. Uninoculated bioreactors inhibited with 0.5% Formalin and 0.2% sodium azide retained greater...

Introduction In order to maximize cell growth within a compact space and retain cells for easy medium exchange, the iCELLis bioreactors from ATMI LifeSciences contain macro-carriers trapped in a fixed-bed, creating a 3-D matrix within which cells adhere and replicate. These bioreactors also enable precise temperature, pH and dissolved oxygen control which cannot be done in 2-D cultures. The iCE...

The sources of occurrence of various pollutants from chemical process industries and there harmful effects have been highlighted. Typical composition of wastewater from various sources presented. The methods of treatment of wastewater briefly discussed. Special attention has been paid to the biological treatment mentioning the drawbacks of the traditional methods. The relative advantages of var...

Biochemical reactors are essential unit operations in a wide variety of biotechnological processes. As compared to conventional chemical reactors, bioreactors present unique modeling and control challenges due to the complexity of the underlying biochemical reactions and the heterogenous nature of cell populations. The dynamic behavior of bioreactors can be strongly affected by variations betwe...

Bioprocess scale-up is a fundamental component of process development in the biotechnology industry. When scaling up a mammalian cell culture process, it is important to consider factors such as mixing time, oxygen transfer, and carbon dioxide removal. In this study, cell-free mixing studies were performed in production scale 5,000-L bioreactors to evaluate scale-up issues. Using the current bi...

The murine B-lymphocyte hybridoma cell line, CC9C10, was grown in serum-free continuous culture at steady-state dissolved oxygen (DO) concentrations of 10%, 50%, and 100% of air saturation in both LH Series 210 (LH) and New Brunswick Scientific (NBS) CelliGen bioreactors. All culture parameters were monitored and controlled and were nominally identical at steady state in the two bioreactors. Th...