We tested the hypothesis that the mitochondrial form of superoxide dismutase (Mn-SOD) protects the cerebral vasculature. Basilar arteries (baseline diameter ~140 μm) from mice were isolated, cannulated and pressurized in order to measure vessel diameter. In arteries from C57BL6 mice preconstricted with U-46619, acetylcholine (Ach, an endothelium-dependent vasodilator) produced dilation that was...

BACKGROUND Clinical studies suggest that short-term insulin treatment in new-onset type 2 diabetes (T2DM) can promote prolonged glycemic control. The purpose of this study was to establish an animal model to examine such a "legacy" effect of early insulin therapy (EIT) in long-term glycemic control in new-onset T2DM. The objective of the study was to investigate the role of diet following onset...

It is well established that tissue damage and functional outcome after experimental or clinical stroke are shaped by biologic sex. We investigated the novel hypothesis that ischemic cell death from neuronally derived nitric oxide (NO) or poly-ADP ribose polymerase (PARP-1) activation is sexually dimorphic and that interruption of these molecular death pathways benefits only the male brain. Fema...

Bisphenol S is an endocrine disruptor exhibiting metabolic disturbances, especially following perinatal exposures. To date, no data are available on the obesogen effects of BPS in a mutligenerational issue. We investigated multigenerational study by focusing body weight, adipose tissue and plasma parameters male female mice. Pregnant C57BL6/J mice were exposed to (1.5 ?g/kg bw/day ie human equi...

BACKGROUND Nitric oxide (NO) and carbon monoxide (CO) in exhaled breath are considered obtainable biomarkers of physiologic mechanisms. Therefore, obtaining their measures simply, non-invasively, and repeatedly, is of interest, and was the purpose of the current study. METHODS Expired NO (ENO) and CO (ECO) were measured non-invasively using a gas micro-analyzer on several strains of mice (C57...

Sharma N, Li L, Ecelbarger CM. Sex differences in renal and metabolic responses to a high-fructose diet in mice. Am J Physiol Renal Physiol 308: F400–F410, 2015. First published December 23, 2014; doi:10.1152/ajprenal.00403.2014.—High fructose intake has been associated with increased incidences of renal disease and hypertension, among other pathologies. Most fructose is cleared by the portal s...

Abstract Background Obesity‐related asthma, a specific type of tends to have more severe symptoms and frequent exacerbations, it is insensitive standard medications. Plumbagin (PLB) has many positive effects on human health. However, remains unclear whether PLB protects against obesity‐related asthma. The study investigated the effect Methods Four‐week‐old male C57BL6/J mice were fed either sta...

High fructose intake has been associated with increased incidences of renal disease and hypertension, among other pathologies. Most fructose is cleared by the portal system and metabolized in the liver; however, systemic levels of fructose can rise with increased consumption. We tested whether there were sex differences in the renal responses to a high-fructose diet in mice. Two-month-old male ...