BACKGROUND Nitric oxide (NO) is synthesized from L-arginine by a family of enzymes known as nitric oxide synthases. Low concentrations of NO is essential in biology and physiology of spermatozoa, but high amounts of NO is toxic and has negative effects on sperm functions. Moreover, sperm membrane contains high concentrations of polyunsaturated fatty acids that are highly susceptible to oxidativ...

OBJECTIVE To evaluate the biochemical basis of adult attention-deficit hyperactivity disorder (A-ADHD), we compared lipid peroxidation status in the plasma of A-ADHD patients, and that of control subjects without A-ADHD by quantifying the levels of malondialdehyde (MDA), an end product of fatty acid oxidation. We aimed to examine the association between MDA and A-ADHD. METHOD The study compri...

We investigated the effect of ciprofloxacin, rifampicine and doxycycline on myeloperoxidase (MPO) activity, glutathione (GSH) and malondialdehyde (MDA) levels in allergic asthma patients and healthy volunteers. Polymorphonuclear leukocytes (PMNs) were isolated with ficoll-hypaque gradient centrifugation method. MPO activity was assayed with modified o-dianisidine, GSH by Ellman's and MDA levels...

OBJECTIVE Malondialdehyde (MDA) is an end-product formed during lipid peroxidation, due to degradation of cellular membrane phospholipids. MDA is released into extracellular space and finally into the blood; it has been used as an effective biomarker of lipid oxidation. High circulating levels of MDA have been previously described in patients with ischemic stoke than in controls, and an associa...

Malondialdehyde (MDA) is a naturally occurring product of lipid peroxidation; it can also be generated during prostaglandin biosynthesis in cells. MDA reacts with amino groups on proteins and other biomolecules to form a variety of adducts, including adducts with DNA bases that are mutagenic and possibly carcinogenic. Increased levels of lipid peroxidation products, by measurement of MDA, have ...

Background: Psoriasis is a chronic inflammatory skin disease characterized by pathological lesions due to various exogenous and endogenous factors. Oxidative stress can be one of the causes for occurrence psoriasis as well significant contributor its progression. Skin exposure number irritants or proinflammatory agents including UVA UVB generates ROS through oxidative burst in infiltrating leuk...

Numerous experimental and clinical analyses of biomechanical injury have served to elucidate the pathophysiologic events involved in Traumatic Brain Injury(TBI).Secondary insults activate the release of cellular mediators including proinflammatory cytokines,prostaglandins, free radicals,and complement.The resulting cascade of events avalanche into free radical induced oxidative damage to the br...

Polyunsaturated fatty acids have been reported to enhance the cytotoxic activity of several anticancer drugs. In the present study, we observed that doxorubicin chemosensitization of breast cancer cell lines by docosahexaenoic acid (DHA, a long chain omega-3 polyunsaturated fatty acid) was cell-line selective, affecting MDA-MB-231 and MCF-7dox (a doxorubicin resistant cell line) but not the par...

The role of Tumor Necrosis Factor alpha, Malondialdehyde and serum Iron in inducing anemia in Tuberculosis patients was studied. The present study was conducted in the Department of Biochemistry, Shri Aurobindo Institute of Medical Sciences, Indore. The study comprised of sixty-eight anemic pulmonary tuberculosis patients. Levels of Hemoglobin, Iron, TIBC, Ferritin, MDA & TNF α were analyzed in...