To promote studies of cytochrome c (Cyt c) ranging from apoptosis to protein folding, a system for facile mutagenesis and high-level expression is desirable. This work used a generally applicable strategy for improving yields of heterologously expressed protein in Escherichia coli. Starting with the yeast Cyt c plus heme lyase construct of Pollock et al. [Pollock, W. B., Rosell, F. I., Twitchet...

863-Pos Board B632 Hydrogen Bond Strength Modulates the Mechanical Strength of FerricThiolate Bonds in Rubredoxin Peng Zheng, Shin-ichi J. Takayama, A Grant Mauk, Hongbin Li. University of British Columbia, Vancouver, BC, Canada. It has long been recognized that hydrogen bonds formed by protein backbone amides with cysteinyl Sulfur atoms play important roles in modulating the functional and str...

The /3 chain amino terminus of one or more hemoglobins in the blood of members of the Felidae family is substituted with an acetyl group. Such acetylated hemoglobins occur together with nonacetylated components in variable proportions in blood of d8erent members of the family. In the lion, tiger, and snow leopard, the acetylated component comprises about 90% of the total hemoglobin; in the serv...

KASTIN, A. J., M. D. MAUK, A. V. SCHALLY AND D. H. COY. Unusual dose-related effecr of an endorphin a n d o g in u complex maze. PHYSIOL. BEHAV. 25(6) 95S962, 1980.-Hungry adult rats were tested in a 12-choice maze for their ability to find a reward of food I5 minutes after being injected peripherally with (D-Ala‘)-D-endorphin. Injection of the endorphin analog in a dose of 80 &kg body weight r...

Certain materials used in lasers are made by a process called epitaxial semiconductor crystal growth. In this report a mathematical model is developed for this growth process which occurs on a substrate at the junction between a masked region and exposed substrate in a vapor. This new model consists of two partial differential equations; one for the surface dynamics and one for the crystal grow...