Abstract Background Triple-action diazabicyclooctanes, e.g. zidebactam, combine ?-lactamase inhibition, antibacterial activity, and ‘enhancement’ of PBP3-targeted ?-lactams. Objectives To examine the activity cefepime/zidebactam against consecutive ‘problem’ Gram-negative bacteria referred to UK national reference laboratory. Methods MICs were determined by BSAC agar dilution for 1632 Enterobac...

BACKGROUND & OBJECTIVE The production of carbapenemases is an important mechanism responsible for the carbapenem resistance. A simple and inexpensive testing method for screening of carbapenemase producers is essential. A prospective study was undertaken to detect metallo-beta-lactamases (MBLs) and AmpC beta-lactamases in nonfermentative Gram negative bacteria and to evaluate the various method...

BACKGROUND Production of β-lactamase enzymes is the most common and important mechanism of resistance in Gram-negative bacteria. The objective of this study was to assess frequency of three main β-lactamase enzymes, including extended spectrum β-lactamases (ESBLs), metallo-β-lactamase (MBL), and Klebsiella pneumoniae carbapenemase (KPC) enzymes in Escherichia coli and Klebsiella spp. isolated f...

BACKGROUND Nosocomial infections caused by Pseudomonas aeruginosa presenting resistance to beta-lactam drugs are one of the most challenging targets for antimicrobial therapy, leading to substantial increase in mortality rates in hospitals worldwide. In this context, P. aeruginosa harboring acquired mechanisms of resistance, such as production of metallo-beta-lactamase (MBLs) and extended-spect...

INTRODUCTION We hypothesized that extravascular body fluid (EBF) analysis in Croatia is not harmonized and aimed to investigate preanalytical, analytical and postanalytical procedures used in EBF analysis in order to identify key aspects that should be addressed in future harmonization attempts. MATERIALS AND METHODS An anonymous online survey created to explore laboratory testing of EBF was ...

Metallo-β-lactamases (MBLs) have rapidly disseminated worldwide among clinically important Gram-negative bacteria and have challenged the therapeutic use of β-lactam antibiotics, particularly carbapenems. The bla(GIM-1) gene, encoding one such enzyme, was first discovered in a Pseudomonas aeruginosa isolate from 2002 and has more recently been reported in Enterobacteriaceae. Here, we present cr...

In Gram-negative bacteria, resistance to β-lactam antibacterials is largely due to β-lactamases and is a growing public health threat. One of the most concerning β-lactamases to evolve in bacteria are the Class B enzymes, the metallo-β-lactamases (MBLs). To date, penams and cephems resistant to hydrolysis by MBLs have not yet been found. As a result of this broad substrate specificity, a better...

| 2017 | 1 Centre for AIDS Research and Education (YRG CARE), South India. The Gram‐negative bacterial isolates were screened for ESBLs production using cefotaxime (30 μg) and ceftazidime (30 μg) alone and in combination with clavulanic acid (10 μg), for MBL production using imipenem (10 μg) alone and in combination with ethylenediaminetetraacetic acid (EDTA) (750 μg), and for AmpC production u...

Carbapenemases production, either metallo-lactamases (MBLs), KPC or oxacillinases (OXA), is the main resistance mechanism responsible for resistance phenotype to carbapenems in Acinetobacter.1 While for MBLs and KPCs there are some screening tests for their detection,2,3 the same is not true for oxacillinases. During the investigation of a large outbreak, we analyzed 584 carbapenem-resistant Ac...

results among the 225 isolates, 33.7% (76/225) and 18.1% (41/225) were resistant to imipenem and meropenem, respectively. of the 76 imipenem-resistant p. aeruginosa strains, 45 (59.2%) were positive for mbls, 34 (75%) strains carried the blaimp-1 gene, and 1 (2.2%) strain carried the blavim-2 gene. conclusions our results showed that there was a high frequency of imp-1 positive p. aeruginosa in...