cellulase production by the fungus trichoderma reesei was studied using sugar beet pulp (sbp) as a substrate. the subculture medium was a salt solution consisting of kh2po4, cacl2, etc. fungal cells were sub-cultured in an orbital shaker (180 rpm) at 30°c for 1-2 generations (two days for each generation) and were then used as an inoculum. exponential cells were inoculated into a medium contain...
