The intentional adulteration of pet food with melamine and cyanuric acid has been implicated in the kidney failure and death of a large number of cats and dogs in the United States. Although individually these compounds present low toxicity in a range of experimental animals, coexposure can lead to the formation of melamine cyanurate crystals in the nephrons and eventual kidney failure. Given t...

OBJECTIVE To develop an analytical method for simultaneously qualitative and quantitative determination of melamine and triazine-related by-products including ammelide, ammeline, and cyanuric acid in milk and milk products by gas chromatography-tandem mass spectrometry (GC-MS/MS). METHODS Melamine and triazine-related by-products namely ammelide, ammeline and cyanuric acid in the samples were...

1. The degradative pathway of melamine (1,3,5-triazine-2,4,6-triamine) was examined in Pseudomonas sp. strain A. 2. The bacterium grew with melamine, ammeline, ammelide, cyanuric acid or NH+4 as sole source of nitrogen, and each substrate was entirely metabolized. Utilization of ammeline, ammelide, cyanuric acid or NH+4 was concomitant with growth. But with melamine as substrate, a transient in...

Cyanuric acid is able to form complementary hydrogen bonds with melamine. Here, the specific recognition between cyanuric acid and melamine is used to significantly improve the gene transfection efficacy of low generation dendrimers via a supramolecular approach.

Coumarins and their derivatives are important classes of heterocyclic compounds whose synthesis has been the focus of attention of many organic and medicinal chemists. Such an interest in these compounds can be attributed to their variety of bioactivities such as, inhibition of platelet aggregation [1], antibacterial [2], anticancer [3], inhibitor of steroid 5αreductase [4] and inhibitor of HIV...

Although the toxicity of melamine to the kidneys and testes is well known, few studies have investigated the effects of melamine on female reproductive organs. Therefore, this study explores the effects of oral administration melamine or melamine and cyanuric acid for 28 days on the ovaries of female rats. Rats that were exposed to the mixture exhibited reduced ovarian and uterine weights, a sh...

Sulfonic aromatic compounds are widely used in the textile industry for the synthesis of azo dyes. The presence of the sulfonic group endows these compounds with high water-solubility and resistance to biological treatment. The present work analysed the efficacy of oxidation with ozone in the treatment of waters containing naphthalene-1-sulfonic acid, naphthalene-1,5-disulfonic acid and naphtha...

OBJECTIVES To study the migration of melamine into foods from plastic food packaging materials and dairy product containers commonly used in China. METHODS 37 samples were collected from the market. The EU migration testing conditions were adopted with distilled water, 3% acetic acid, n-hexane and 15% ethanol being chosen as the simulating solutions. The HPLC method was used to detect melamin...

Rhodococcus sp. strain Mel was isolated from soil by enrichment and grew in minimal medium with melamine as the sole N source with a doubling time of 3.5 h. Stoichiometry studies showed that all six nitrogen atoms of melamine were assimilated. The genome was sequenced by Roche 454 pyrosequencing to 13× coverage, and a 22.3-kb DNA region was found to contain a homolog to the melamine deaminase g...

The gene encoding melamine deaminase (TriA) from Pseudomonas sp. strain NRRL B-12227 was identified, cloned into Escherichia coli, sequenced, and expressed for in vitro study of enzyme activity. Melamine deaminase displaced two of the three amino groups from melamine, producing ammeline and ammelide as sequential products. The first deamination reaction occurred more than 10 times faster than t...