BACKGROUND KRAS mutational analysis is the standard of care prior to initiation of treatments targeting the epidermal growth factor receptor (EGFR) in patients with metastatic colorectal cancer. Sensitive methods are required to reliably detect KRAS mutations in tumor samples due to admixture with non-mutated cells. Many laboratories have implemented sensitive tests for KRAS mutations, but the ...

conclusions according to the factory noise map, except for the control rooms with 81 dba noise, other cases of measuring stations were higher than the standard exposure of 85 dba noise. as a result, the implementation of control strategies, such as inspection and performance of control systems and insulation of the control room to prevent unnecessary exposure noise, are necessary. background st...

Incidence of parasitism is often underestimated because ‘ghost’ parasitoids (dead unemerged or those that have emerged leaving the host carcasses) are difficult to detect and identify. This study demonstrates use melting curve analysis (MCA) carcasses can identify DNA ghost even a month after death. The coccinellid hosts Cycloneda sanguinea, Eriopis connexa, Harmonia axyridis Hippodamia converg...

Following the initial report of the use of SYBR Green I for real-time polymerase chain reaction (PCR) in 1997, little attention has been given to the development of alternative intercalating dyes for this application. This is surprising considering the reported limitations of SYBR Green I, which include limited dye stability, dye-dependent PCR inhibition, and selective detection of amplicons du...

Zambounis A., Samaras A., Xanthopoulou A., Osathanunkul M., Schena L., Tsaftaris A., Madesis P. (2016): Identification of Phytophthora species by a high resolution melting analysis: an innovative tool for rapid differentiation. Plant Protect. Sci., 52. A new molecular method via the high resolution melting (HRM) analysis of the Ypt1 gene non-coding regions was validated for ten Phytophthora spe...

Rice (Oryza sativa L.) is one of the most important crops in the world. It is essential to develop an efficient method to build the fingerprinting database of commercial hybrid rice cultivars and their parental lines in seed market for rapid and unambiguous cultivar identification and seed purity analysis as new similar cultivars increasing yearly. The objective of this investigation were to fi...

BACKGROUND Combination of CHD (chromo-helicase-DNA binding protein)-specific polymerase chain reaction (PCR) with electrophoresis (PCR/electrophoresis) is the most common avian molecular sexing technique but it is lab-intensive and gel-required. Gender determination often fails when the difference in length between the PCR products of CHD-Z and CHD-W genes is too short to be resolved. RESULTS...