The genetic diversity and phylogenetic relationships of eight different varieties of Rice (Oryza sativa) viz., MO 16, Ptb 43, Palakkaddan Matta, Ptb 40, MO 17, MO 6, Ptb 39 and KTR 2 from Kerala state, South India were analyzed by Random amplified polymorphic DNA (RAPD). Out of twenty 10-mer RAPD primers screened initially, eleven were chosen and used in a comparative analysis of different vari...

Twenty nine samples of pigeon droppings (n = 12) and soil contaminated with avian excreta (n = 19), collected from different sites in Busan, were examined for isolation and characterization of Cryptococcus neoformans. Of these samples, 5 strains of C. neoformans were recovered from pigeon droppings (5/12 : 41.7%). All isolates were belonged to C. neoformans var. grubii (serotype A). The extrace...

Plants intermediate in appearance between Nuphar microphyllaand N. variegata (Nymphaeaceae) have long been assumed to bethe result of hybridization. The evidence for this is based primarilyon field observations of morphology, poor fruit production, closegeographical proximity of presumed parent species, and limited pollensterility data. Fertile populations of the same plants have also beendocum...

Random amplification of polymorphic DNA (RAPD) analysis using the polymerase chain reaction proved a useful technique in the biodiversity analysis of microorganisms but may lack reproducibility in the poorly standardized methodology. In the present investigation, the RAPD technique was optimized for characterizing cyanobacterial isolates in order to ensure its reproducibility and discriminatory...

A rapid and efficient procedure for site specific mutagenesis is described. A double primed synthesis with a 17-mer mismatch primer and a "universal" 15-mer M13 sequencing primer was used to introduce a T to A transversion into an ompF signal peptide gene cloned in the M13mp8 vector. The two primers were annealed to the circular single stranded M13 template. After a short extension and ligation...

synthetic plants as well as wild crop species are valuable genetic resourses. to determine the genetic variation in triticale, tritipyrum as well as wheat lines, genomic dna of this amphiploid including thinopyrum bessarabicum and durum wheat were amplified using 32 random and 22 semi random primers. primers that could produce clear, polymorphic and repetitive bands are used for determination o...

genetic diversity of 22 morphotypes of diploid wheat (t. urartu) was investigated using morphological traits and rapd markers. morphological characterizations were carried out based on 17 traits and statistical parameters of dispersion and diversity of agronomic traits were calculated. statistical analysis showed significant differences for all characters among morphotypes. dendrograms were gen...

Single-stranded long oligonucleotide-based (50- to 70-mer) microarrays offer several advantages over conventional cDNA microarrays. These include the easy preparation of the probes, low cost of array production, and low cross-contamination during probe handling. However, the application of oligonucleotide microarrays for the analysis of global gene expression with small amounts of total RNA usi...

Figure S1: Regmex p-value output for different scenarios of sequence lengths, number of sequences and motif enrichements. (A) Random sequences (n=100) of length 100 nucleotides with a number of 7-mer motif (AATGCCC) inserted among the leading 30 sequences as indicated along the x-axis. (B) Random sequences (n=10,000) of length 100 nucleotides with a number of 7-mer motif (AATGCCC) inserted amon...

saffron (crocus sativus) is the most valuable and indigenous crop in iran. the stigmas of flower are used as a popular natural flavouring, colouring and medicinal agent. however, the market suffers from frauds in this plant such as mixing with safflower petals due to high profit. identification of these frauds with conventional and biochemical methods is difficult and low sensitive. therefore, ...