Staphylococcus aureus is a common cause of hospital-acquired infections. This bacterium is resistant to β-lactam antibiotics. The aim of this study was to evaluate the prevalence of S. aureus strains producing enzymes capable of destroying β-lactam antibiotics in a hospital environment. This descriptive and analytic study was performed in Besat Hospital, Sanandaj, Iran, 2016, on 158 swabs colle...

An Ambler class A β-lactamase gene, bla(CIA-1), was cloned from the reference strain Chryseobacterium indologenes ATCC 29897 and expressed in Escherichia coli BL21. The bla(CIA-1) gene encodes a novel extended-spectrum β-lactamase (ESBL) that shared 68% and 60% identities with the CGA-1 and CME-1 β-lactamases, respectively. bla(CIA-1)-like genes were detected from clinical isolates. In addition...

Carbapenem resistance caused by metallo-β-lactamases is a serious global challenge that, if not tackled efficiently, expected to lead millions of deaths in the coming decades. Verona-integron encoded metallo-β-lactamase 2 (VIM-2) bacterial enzyme that has been reported from multidrug-resistant nosocomial isolates Pseudomonas aeruginosa and other Gram-negative pathogens. As it hydrolyzes most β-...

Carbapenemases increasingly have been reported in Enterobacteriaceae in the past 10 years. Klebsiella pneumoniae carbapenemases have been reported in the United States and then worldwide, with a marked endemicity at least in the United States and Greece. Metallo-enzymes (Verona integron-encoded metallo-β-lactamase, IMP) also have been reported worldwide, with a higher prevalence in southern Eur...

BACKGROUND We evaluated the combined use of the modified Hodge test (MHT) and carbapenemase inhibition test (CIT) using phenylboronic acid (PBA) and EDTA to detect carbapenemase-producing Enterobacteriaceae (CPE) and metallo-β-lactamase (MBL)-producing Pseudomonas spp. METHODS A total of 49 isolates of CPE (15 Klebsiella pneumoniae carbapenemase [KPC], 5 Guiana extended-spectrum β-lactamase [...

MTH1203, a β-CASP metallo-β-lactamase family nuclease from the archaeon Methanothermobacter thermautotrophicus, was identified as a putative nuclease that might contribute to RNA processing. The crystal structure of MTH1203 reveals that, in addition to the metallo-β-lactamase nuclease and the β-CASP domains, it contains two contiguous KH domains that are unique to MTH1203 and its orthologs. RNA...

New Delhi metallo-β-lactamase (NDM-1) is a new metallo-β-lactamase (MBL) that has recently emerged as a global threat because it confers bacteria with resistance to almost all clinically used β-lactam antibiotics. To determine the molecular basis of this threat, NDM-1 was purified from Escherichia coli TransB (DE3) carrying cloned blaNDM-1 gene by an anion-exchange chromatography step followed ...

Introduction In this era of modern medicine, antimicrobial resistance can be regarded as a major health calamity. The emergence multidrug-resistant (MDR) Pseudomonas aeruginosa strains poses therapeutic challenges and lead to treatment failure in hospitalized patients. This study was conducted determine various types β-lactamases among MDR P. isolates recovered from patients.
 MethodsThis ...

conclusions a high prevalence of multiple β-lactamase production was observed among the ampc producers (60%), of which the majority co-produced ampc and mbl. the current study results showed correlation between β-lactamase production and the presence of antibiotic resistance genes in the isolates. results all isolates were multidrug resistant. ampc, esbl and mbl production were observed in 35 (...