In the study of 782 biopsy specimens from 1 95 patients during and after chemotherapy we compared the numbers of Mycobacterium /eprae stained by the Fite-Faraco (FF) and the Gomori methenamine-silver (GMS) techniques. In many patients large amounts of non-acid-fast M. /eprae or remnants thereof remained 66 months after starting effective multidrug therapy. The GMS stain is a useful method for a...

Reduced acid-fast staining of leprosy bacilli occurs during the dewaxing of paraffin sections by xylene and alcohols; the older and more decrepit bacilli being especially affected. By the use of non-deparaffinized sections, the leprosy bacilli which could not be stained with the usual carbol fuchsin are strongly stained. Moreover, non-deparafinized sections can be used for the periodic acid-car...

Placental estriol in urine can be determined by rapid enzymatic hydrolysis, extraction with neutral XAD-2 resin, and quantitation by gas-liquid chromatography, with equilenin as the internal standard. Glucose, methenamine mandelate, hydrochlorothiazide, phenolphthalein, or salicylate do not interfere. Analytical recovery of estriol added to urine was 92-108%. Day-to-day precison (CV), establish...

We describe a radioimmunoassay for total urinary estriol in pregnancy. A 25-mul aliquot of the urine specimen is acid hydrolyzed, neutralized, and diluted before assay. We use rabbit antisera against estriol-6-(O-carboxymethyl)oxime/bovine serum albumin and ammonium sulfate precipitation at room temperature. Results are unaffected by glucose or methenamine mandelate, a urinary tract antiseptic....

We are reporting a rare case of primary mucocutaneous histoplasmosis. A 47-year-old male with a part-time job of rearing pigeons presented with a non-healing oral ulcer with no associated systemic symptoms. Differential diagnosis of oral ulcerative lichen planus, squamous cell carcinoma and mucocutaneous leishmaniasis was considered. Histopathology showed multiple organisms with a peripheral ha...

This study reports the epidemiological, pathological and mycological findings of cutaneous pythiosis in cattle in southern Brazil. 23 calves, that were kept next to a river with extensive marshy regions, presented ulcerated cutaneous lesions in thoracic and pelvic limbs, sometimes extending to the ventral thoracic region. Histopathological examination revealed multifocal pyogranulomas in the su...

Figure 1. A, Grocott methenamine silver (GMS) stain showing Pneumocystis organisms within a granuloma. Inset, digitally magnified Pneumocystis organism showing characteristic paired “dark bodies” diagnostic of Pneumocystis species. B, Immunohistochemical stain using antibodies directed against Pneumocystis species with use of the treptavidin-biotin immunoperoxidase method. The discreet nature o...

Microbial resistance in livestock has become a subject of great concern public and scientific interest. This study was designed to assess the effects methenamine feeding regime on growth performances broilers chickens. For this purpose, 120 chicks Cobb 500 strain, including 60 males females 21 days old with an average weight 639 g 584 respectively were used. They randomly distributed experiment...