We developed multiplex polymerase chain reaction (PCR) to detect aac(6 ')/aph(2 "), aph(3 ')-IIIa, and ant(4 ')-Ia, the genes encoding the most clinically relevant amino-glycoside modifying enzymes (AME), and simultaneously, the methicillin resistant gene, mecA, in Staphylococcus species. Clinical isolates of 45 S. aureus and 47 coagulase negative staphylococci (CNS) from tertiary university ho...

The most prevalent community-associated methicillin-resistant Staphylococcus aureus (C-MRSA) strains in Taiwan, sequence type 59 (ST59) clones, carry staphylococcal cassette chromosome mec (SCCmec) type V and, to a lesser extent, type IV. These strains show wide variation in sensitivity to oxacillin, but the reasons for this variation are unknown. Here we compared the sequences of the mecA gene...

coagulase-negative staphylococci (cons) are often associated with bovine mastitis and may be resistant to antimicrobial therapy. the aim of the current study was to investigate the presence of blaz (responsible for penicillin resistance) and meca (responsible for methicillin resistance) genes among 108 cons belonging to 9 different species isolated from bovine mastitis in seven dairy herds (h1-...

background : infections resulted from multi-resistant staphylococcus aureus are increasing worldwide. in the present study, a multiplex-pcr assay for the detection of antibiotic resistance genes among s. aureus clinical isolates and for the concomitant identification of these isolates was described. methods : a total of 127 s. aureus isolates were collected from clinical specimens at three teac...

The aim of this study was to examine phenotypic and genotypic antimicrobial resistance of staphylococci from milk samples from cows with subclinical and clinical mastitis and from cows without mastitis symptoms to methicillin, tetracyclines, macrolides and lincosamides (ML). Of 207 strains, including 34 S. aureus and 173 coagulase-negative staphylococci (CNS), 11 (6.4%) CNS strains were phenoty...

Strains of methicillin-resistant Staphylococcus aureus (MRSA) have become the most important causative agents of hospital-acquired diseases worldwide. The genetic determinant of resistance, mecA, is not a gene native to S. aureus but was acquired from an extraspecies source by an unknown mechanism. We recently identified a close homologue of this gene in isolates of Staphylococcus sciuri, a tax...

The identification of methicillin-resistant staphylococcus isolates in the clinical laboratory has typically been performed by using methods that detect phenotypic expression of resistance determinants. However, these methods may be difficult to interpret and some isolates do not express resistance until selective pressure is administered. Assays that detect genetic determinants are not subject...

BACKGROUND AND OBJECTIVES Staphylococcus aureus is one of the main causatives of bovine mastitis. Resistance of some strains to methicillin, can complicate the treatment of its infections. On the other hand, enterotoxin production is also important. Therefore, the aim of our study was to investigate the methicillin resistance and enterotoxin production in S. aureus isolates caused bovine mastit...

background: methicillin resistant staphylococcus aureus (mrsa) is one of the major agents for increasing number of serious hospital and community acquired infections. the aim of this study was to investigate the occurrence of the mrsa and meca gene among nosocomial and environmental specimens in kurdistan hospitals and determining the antibiotic resistance of the isolates. materials and methods...

Abstract Staphylococcus aureus is an important foodborne pathogen associated to food intoxication and other multiple infections in human being. Its presence salted a serious issue due its salt tolerance potential. A study was conducted analyze the of enterotoxins producing drug resistance S. sea fish from Gwadar. Freshly persevered samples (n=50) were subjected using 16S rRNA Nuc genes primers....