Bioactivity-directed fractionation of the fermentation broth and mycelium of the coprophilous fungus Guanomyces polythrix led to the isolation of several phytotoxic compounds, including five new naphthopyranone derivatives (1-5). In addition, rubrofusarin B, emodin, citrinin, and 4-hydroxybenzoic acid methyl ester were obtained. The structures of the new compounds were established by spectral a...

In this article we describe development and validation of stability indicating, accurate, specific, precise and simple Ion-pairing RP-HPLC method for simultaneous determination of paracetamol and cetirizine HCl along with preservatives i.e. propylparaben, and methylparaben in pharmaceutical dosage forms of oral solution and in serum. Acetonitrile: Buffer: Sulfuric Acid (45:55:0.3 v/v/v) was the...

A stability-indicating reversed-phase high-performance liquid chromatography (RP-HPLC) method was developed for the simultaneous determination of halometasone, fusidic acid, methylparaben, and propylparaben in topical pharmaceutical formulation. The desired chromatographic separation was achieved on an Agilent Zorbax CN (Cyano), 5 μm (250 × 4.6 mm) column using gradient elution at 240 nm detect...

A simple, fast, and efficient RP-HPLC method has been developed and validated for the simultaneous estimation of Levodropropizine, Chloropheniramine, Methylparaben, Propylparaben, and the quantification of Levodropropizine impurities in the Reswas syrup dosage form. A gradient elution method was used for the separation of all the actives and Levodropropizine impurities by using the X-Bridge C18...

MARCAINE with epinephrine 1:200,000 (as bitartrate)—Sterile isotonic solutions containing sodium chloride. Each mL contains bupivacaine hydrochloride and 0.0091 mg epinephrine bitartrate, with 0.5 mg sodium metabisulfite, 0.001 mL monothioglycerol, and 2 mg ascorbic acid as antioxidants, 0.0017 mL 60% sodium lactate buffer, and 0.1 mg edetate calcium disodium as stabilizer. In multiple-dose via...

ovarian cancer is one of the major causes of death in women. parabens are a class of chemicals widely used as preservatives in cosmetic and pharmaceutical products, they mimic estrogens, which are known to play a role in the development of different cancers in women. the aim of this study was to investigate the proliferative effects of methyl paraben (mp) and propyl paraben (pp) on human ovaria...

Dermal permeation through human epidermis and uptake into isolated human stratum corneum (SC) that was and was not delipidized were measured for 2 model compounds, 4-cyanophenol (CP) and methyl paraben (MP), from saturated aqueous solutions containing 1 or both compounds. Because the solutions were in equilibrium with the pure CP and MP, the thermodynamic activity of the compounds was constant....

Abstract Gentamicin sulfate is a potent broad spectrum aminoglycoside antibiotic which used against Gram-positive and Gram-negative bacteria. A simple, isocratic HPLC method for separation, identification determination of gentamicin parabens (methylparaben propylparaben) was developed validated. To our knowledge there no report about simultaneous those three analytes in pharmaceutical products....

This paper describes the methodological optimization and validation of a simple micellar electrokinetic chromatography for the rapid simultaneous separation of 2-methyl-4-isothiazolin-3-one, methylparaben, ethylparaben, propylparaben, and butylparaben. By using a 30 mM phosphate buffer (pH 7.2) containing 30 mM sodium dodecyl sulfate (SDS), an applied voltage of 10 kV and a separation temperatu...