ECM protein enrichment and immunoblotting 100mg of frozen samples were homogenized using a polytron (Kinematica, Bohemia, NY) in 250μL of buffer C (HEPES pH7.9, MgCl2, KCl, EDTA, Sucrose, Glycerol, Sodium OrthoVanadate) of the CNMCS protein extraction kit. Lysates were then spun for 20 min. at 15,000 rpm at 4°C, the supernatant was saved (intermediate fraction 1, enriched for cytosolic proteins...

RETURN TO ISSUEPREVAddition/CorrectionNEXTORIGINAL ARTICLEThis notice is a correctionCorrection to “Elucidating Ionic Correlations Beyond Simple Charge Alternation in Molten MgCl2–KCl Mixtures”Fei WuFei WuMore by Fei Wu, Santanu RoySantanu RoyMore Royhttp://orcid.org/0000-0001-6991-8205, Alexander S. Ivanov*Alexander Ivanov*[email protected]More Ivanovhttp://orcid.org/0000-0002-8193-6673, Simer...

A calorimetric titration method was used to study the ADP binding to the chymotryptic subfragments of myosin, heavy meromyosin (HMM) and myosin subfragment 1 (S-1), and to myosin aggregated into filaments at low ionic strength. The binding constant (K) and heat of reaction (deltaH, kiloJoules (moles of ADP bound)-1) were determined. For HMM in 0.5 M KCl, 0.01 M MgCl2, 0.02 M Tris (pH 7.8) at 12...

Resting membrane potentials, electrical resistance, and coupling of chick retinal pigment epithelium cells in tissue culture have been measured with micropipette electrodes. Topical application of NaCl, KCl, MgCl2, and CaCl2 produce rapid, reversible changes in the membrane potentials of these cells. CaCl2 uniquely induces a slow hyperpolarizing wave that can lead to barrages of depolarizing ac...

The steady-state dissolution rates of basaltic glass and labradorite were measured in the presence 10 to 700 × 10−3 mol·kg−1 aqueous NaCl, KCl, CaCl2, MgCl2 at 25 °C. All mixed flow reactors, pH~3.6 by addition HCl reactive fluids. rates, based on Si release, increased ~0.3 log units either CaCl2 or compared their NaCl KCl. In contrast, decreased ~0.4 These contrasting behaviours likely reflect...

Ionic conducting polymers are of considerable interest recently, as they have wide range applications including electrical storage devices. The present study is aimed at designing and characterizing one such ion polymer complexed with various ionic salts. Here, solid electrolyte films consisting Poly methyl methacrylate (PMMA) chloride salts (X) (X= KCl, NaCl, MgCl2) been prepared by the solven...