The microbial flora of the nose, throat, and feces of male beagle dogs housed in a "locked environment" (i.e. confined to germfree-style isolators and supplied with sterile food, air, and water) or an open environment were assessed between 26 and 30 months into the study. Forty-five genera and 170 different species or types of microorganisms were cultured from the nose, throat, and feces of the...

There is little direct literature detailing exhaustive bacteriological studies comparing human donor faecal flora, human flora-associated (HFA) mouse models and conventional rodent faecal flora. While there is a premise that the implanted donor faecal flora from humans is established in the rodent model the evidence is incomplete and indeed for groups such as Bifidobacterium spp. it is lacking....

Germfree rats were used in 3 experiments to study the effects of the microbial flora on survival time after acute uremia produced by a one-stage bilateral nephrectomy. Germfree rats, limited-flora rats, and conventionalized rats (all maintained continuously in isolators) were subjected to nephrectomy or to sham nephrectomy, deprived of food and water until they died, respectively, of uremia or ...

The human endogenous intestinal microflora is an essential "organ" in providing nourishment, regulating epithelial development, and instructing innate immunity; yet, surprisingly, basic features remain poorly described. We examined 13,355 prokaryotic ribosomal RNA gene sequences from multiple colonic mucosal sites and feces of healthy subjects to improve our understanding of gut microbial diver...

The subgingival bacterial flora from 2 gingival sites was cultured and characterized monthly in twenty periodontitis-free women during pregnancy and again post-partum. Monthly plaque samples were also cultured in eleven age and disease matched non-pregnant women. Plaque was processed anaerobically on selective and nonselective media and the predominant colony types were characterized. A portion...

The microbial flora of the vagina and cervix was assessed qualitatively and semiquantitatively in 40 women attending an intrauterine contraceptive device clinic. Both sites harboured many types of macroorganism, the mean number of microbial types isolated being five from the vagina and four from the cervix. Typical lactobacilli were detected in 61% of vaginal and in 53% of cervical specimens; f...

The microbial flora associated with carcinoma of the oesophagus was studied in 12 patients. Oesophageal mucosa was biopsied at thoracotomy and cultured under both aerobic and anaerobic conditions. A heavy mixed growth of aerobic and anaerobic organisms was obtained in all patients. One-third of isolates were anaerobic. The flora was of oral origin. The most appropriate antibiotic combination in...

This survey was set up on a statistical sampling plan to determine the microbial quality of almonds as they are received at the processing plant. The total aerobic bacterial count and yeast and mold count distribution were skewed by a few high counts compared with the majority of relatively low counts. Hard shell varieties of almonds had lower counts than did soft shell, and almonds with comple...

INTRODUCTION The normal flora can be divided into two groups Resident flora and Transient. Some organisms are almost always present in a normal oral cavity and constitute constant/normal microbial flora. It is further divided into two types: indigenous & supplemental. Comprise of those species that are almost always present in high numbers (> 1%) in a particular site, such as the supra gingival...

Restaurateurs often place a lemon slice on the rim of a beverage glass, or afloat in the beverage, as a flavor-enhancer or a decorative garnish. The handling of the lemons before their placement in the beverage may not follow sanitary procedures. The study reported here investigated whether beverage lemon slices contain microbial contamination that could be consumed by a restaurant patron. Swab...