Microfluidics gained prominence with the application of microelectromechanical systems (MEMS) to chemistry and biology in an attempt to benefit from the miniaturization of devices for handling of minute fluid samples under precisely controlled conditions. The field of microfluidics exploits the differences between microand macro-scale flows, for example, the absence of turbulence, electro-osmot...

We report three novel demonstrations: 1. Coplanar electrowetting 2. Electrowetting on a printed circuit board (PCB) and 3. Transport and mixing in an “open” microfluidic substrate. Similar to “soft” lithography which enabled many researchers to easily prototype and experiment with continuous-flow microfluidics, this work allows researchers to easily experiment with discrete-flow microfluidics (...

Microfluidics is considered an important technology that is suitable for numerous biomedical applications, including cancer diagnosis, metastasis, drug delivery, and tissue engineering. Although microfluidics is still considered to be a new approach in urological research, several pioneering studies have been reported in recent years. In this paper, we reviewed urological research works using m...

Open-surface digital microfluidics refers to droplet manipulations in an open surface. Different chemical/biological reaction protocols can easily be demonstrated in the same device by reprogramming the order of droplet manipulations. While continuous-flow or liquid-plug microfluidics within microchannels have inherently limited programmability, there is no limit of reprogrammability in open-su...

This study presents a novel microfluidic approach for developing large scale screening assays of anticancer compounds on 3D multicellular spheroids. We have developed a microfluidic device with associated protocols that combine the high-throughput characteristics of droplet microfluidics for spheroid formation and aggregation with those of single-phase microfluidics for substance exchange, long...

Microfluidic chips provide unparalleled control over droplets and jets, which have advanced all natural sciences. However, microfluidic applications could be vastly expanded by increasing the per-channel throughput and directly exploiting the output of chips for rapid additive manufacturing. We unlock these features with in-air microfluidics, a new chip-free platform to manipulate microscale li...

This paper reviews the technologies that have been invented in the last few years on high-throughput phenotyping, imaging, screening, and related techniques using microfluidics. The review focuses on the technical challenges and how microfluidics can help to solve these existing problems, specifically discussing the applications of microfluidics to multicellular model organisms. The challenges ...

Microfluidics has recently emerged as a new method of manufacturing liposomes, which allows for reproducible mixing in miliseconds on the nanoliter scale. Here we investigate microfluidics-based manufacturing of liposomes. The aim of these studies was to assess the parameters in a microfluidic process by varying the total flow rate (TFR) and the flow rate ratio (FRR) of the solvent and aqueous ...

Microfluidics, a field that has been well-established for several decades, has seen extensive applications in the areas of biology, chemistry, and medicine. However, it might be very hard to imagine how such soft microfluidic devices would be used in other areas, such as electronics, in which stiff, solid metals, insulators, and semiconductors have previously dominated. Very recently, things ha...

We introduce microfluidics technologies as a key foundational technology for synthetic biology experimentation. Recent advances in the field of microfluidics are reviewed and the potential of such a technological platform to support the rapid development of synthetic biology solutions is discussed.