this study investigated genetic variation within indigenous chicken of khorasan using four microsatellite markers (mcw5, mcw16, mcw18 and mcw34). 90 blood samples were collected from this population and phenol-chloroform extraction method was used to isolate the dna. the highest and the lowest allele number and effective alleles are shown in mcw5 (6 and 5.293) and mcw34 (4 and 3.138), respectiv...

one of the traits of the greatest financial impact on sheep production is the number of lambs weaned per ewe with fecb considered as the most well known major gene concerned with this trait. in order to conduct a marker assisted introgression (mai) program the presence of fecb responsible mutation in afshari sheep breeding flock was surveyed. direct test to detect fecbb allele was conducted on ...

The mutation process at microsatellite loci typically occurs at high rates and with stepwise changes in allele sizes, features that may introduce bias when using classical measures of population differentiation based on allele identity (e.g., F(ST), Nei's Ds genetic distance). Allele size-based measures of differentiation, assuming a stepwise mutation process [e.g., Slatkin's R(ST), Goldstein e...

We have amplified a (CA)n:(GT)n microsatellite from the TNF promoters of a panel of mouse strains using the polymerase chain reaction. The length of the microsatellites was polymorphic, with eight alleles observed among 15 inbred strains bearing seven distinct H-2 haplotypes, and four outbred strains. In B10 congenic strains, the TNF allele detected by microsatellite polymorphism segregated wit...

PURPOSE The association of ACE I/D polymorphism with changes in LV mass in response to physical training has been observed, but no association has been found with AT1R A1166C polymorphism. We investigated the ACE I/D, AT1R A1166C, and AT1R CA microsatellite polymorphisms genotype distribution in elite athletes and whether the presence of AT1R C1166 variant, in addition to ACE D allele affects t...

Information on the genetic structure of marine species is essential for stock improvement programs. In orderto analyses the genetic diversity of the Hawksbill turtle (Eretmochelys imbricte) by the microsatellite geneticmethod, 64 samples were caught from the beaches located in Kish and Qeshm islands. Polymerase chainreactions (PCR) of genomic DNA extracted from the samples wer...

BACKGROUND Family-based linkage studies, association studies, and studies of tumors have highlighted human chromosome 8q as a genomic region of interest for prostate cancer susceptibility loci. Recently, a locus at 8q24, characterized by both a single nucleotide polymorphism (SNP) and a microsatellite marker, was shown to be associated with prostate cancer risk in Icelandic, Swedish, and U.S. s...

We have determined the mitotic stability of micro- and mini-satellite DNA sequences in SV40-immortalized Werner syndrome (WS) and control fibroblast cell lines. Five microsatellite loci were genotyped in two WS and two control SV40-immortalized fibroblast cell lines and in 154 independent primary or secondary clones derived from these. We used four minisatellite "core" or individual locus probe...

There is a lack of information on how individual microsatellite loci differ with respect to their mutation properties. Such variation will have an important bearing on our understanding of the ubiquitous occurrence of simple repeat sequences in eukaryotic genomes and on deriving proper mutation models that can be incorporated into genetic distance estimates. We genotyped approximately 100 famil...

Directional evolution in microsatellites is the tendency for microsatellites either to increase or to decrease in size over time between populations. We analyzed 99 microsatellite loci in a sample of 193 maize plants representing the entire pre-Columbian range of this crop for evidence of directional evolution. We took advantage of the known phylogeographic history of maize with the independent...