DNA mismatch repair is an important mechanism involved in maintaining the fidelity of genomic DNA. Defective DNA mismatch repair is implicated in a variety of gastrointestinal and other tumors; however, its role in hepatocellular carcinoma (HCC) has not been assessed. Formalin-fixed, paraffin-embedded archival pathology tissues from 46 primary liver tumors were studied by microdissection and mi...

Analysis of microsatellite instability (MI) and loss of heterozygosity (LOH) is recommended for screening patients with sporadic and hereditary malignancies. This study shows an application of a fluorescent hexaplex PCR system for microsatellite typing on A.L.F. DNA Sequencer (Pharmacia Biotech). This technique detects changes in microsatellites providing a time-efficient, reliable and accurate...

the japanese and english white quails are widespread strains and belongs to the galliformes order, phasianidae family, coturnix genus  and japonica species. these birds are likely to be well-adapted to the hard conditions and resistance to diseases as it has attained economic importance as an agricultural species. in the current study, the genetic variation of japanese and english white  quail ...

this study aimed to estimate microsatellite based genetic diversity in two lines (the selected rirs and control line rirc) of rhode island red (rir) chicken. genomic dna of 24 randomly selected birds maintained at central avian research institute (india) and 24 microsatellite markers were used. microsatellite alleles were determined on 6% urea-page, recorded using geldoc system and the samples ...

The Chinese tree shrew (Tupaia belangeri yaoshanensis) has long been proposed to serve as an animal model for studying human diseases. However, its overall genetic diversity and population structure remain largely unknown. In the present study, we investigated the genetic diversity of population microsatellite DNA in wild Tupaia belangeri yaoshanensis. Sixteen microsatellite loci were assessed ...

Age-related alterations of DNA repair could be involved in the accumulation of genetic damage with age. Few data suggest a possible alteration with age of the mismatch repair system, evidenced by the acquisition of microsatellite instability. We aimed to point out a possible implication of this repair system in the accumulation of genetic damage with age. Peripheral blood cell DNA from 226 part...

The Japanese and English White quails are widespread strains and belongs to the Galliformes order, Phasianidae family, Coturnix genus  and Japonica species. These birds are likely to be well-adapted to the hard conditions and resistance to diseases as it has attained economic importance as an agricultural species. In the current study, the genetic variation of Japanese and English White  quail ...

We present the first set of microsatellite markers developed exclusively for an extinct taxon. Microsatellite data have been analysed in thousands of genetic studies on extant species but the technology can be problematic when applied to low copy number (LCN) DNA. It is therefore rarely used on substrates more than a few decades old. Now, with the primers and protocols presented here, microsate...

Microsatellite DNAs that form non-B structures are implicated in replication fork stalling, DNA double strand breaks (DSBs) and human disease. Fanconi anemia (FA) is an inherited disorder in which mutations in at least nineteen genes are responsible for the phenotypes of genome instability and cancer predisposition. FA pathway proteins are active in the resolution of non-B DNA structures includ...