Chinese cabbage buds were soaked with Ethyl methanesulfonate (EMS) to induce mutagenesis. The influence of different EMS concentrations and treatment durations on microspore development, embryo production rate and seedling rate were evaluated in five Chinese cabbage genotypes. Mutations in four color-related genes were identified using high resolution melting (HRM) curves of their PCR products....

To establish a high-efficiency system of isolated microspore culture for different barley genotypes, we investigated the effects of nitrogen sources and concentrations on callus induction and plant regeneration in different barley genotypes. The results showed that the organic nitrogen sources greatly increased the callus induction, and the great reduction of total nitrogen sources would signif...

Abstract The effectiveness of microspore embryogenesis (ME) is determined by a complex network internal and environmental factors. In the present study on triticale barley, strong positive correlation (r = 0.85) between generation hydrogen peroxide (H 2 O ) ME confirmed important role reactive oxygen species in reprogramming. However, for high induction, intensive H had to be associated with ac...

COMPARISON of microsporogenesis and microspore development A in a triploid Tradescantia has shown significant deviations from the normal diploid behavior. Occasional dicentric chromatids a t meiosis are found in diploid Tradescantias, but inversion crossovers in the triploid are much more frequent. An analysis of microspore development in a triploid Tradescantia shows that chromosome number has...

In plant breeding studies, dihaploidization method is used to obtain homozygous pure lines develop varieties in a shorter time. Dihaploidization applied most types of vegetables such as wheat and corn, well vegetable species. Androgenesis methods (anther microspore culture) gynogenesis parthenogenesis (ovula ovary are haploid plants. One the androgenetic methods, shed-microspore culture has bee...

Ovary pre-conditioned medium and ovary co-culture increased the efficiency of green doubled haploid plant production in bread wheat anther culture. The positive effect of this medium led to a 6- and 11-fold increase in the numbers of embryos and green plants, respectively, having a greater effect on a medium-low responding cultivar. Ovary genotype and developmental stage significantly affected ...

Microspores are specialized generative cells with haploid genome that demonstrate the amenability toward embryogenesis under certain conditions. The induced microspore culture technique is largely exploited by the breeding programs of wheat and other crops due to its high efficiency for generation of the large number of haploid plants in the relatively short period of time. The ability to produ...

Male reproductive development in rice (Oryza sativa Linnaeus is very sensitive to various forms of environmental stresses including low temperature. Here, we present our findings on the proteomic analysis of the later developmental consequences of low temperature treatment on rice anthers. Anther proteins at the trinucleate stage, with or without cold treatment for four days at 12 degrees C at ...

The analysis of microsporogenesis in endogamous plants of popcorn (S5 to S7) showed several and distinct interchanges which involve the nucleolus organizer (chromosome 6). The detection of cells with interchanges was facilitated by the presence of two nucleoli of different sizes in contrast to normal ones with a single big nucleolus. Interchange points do not always seem to be at the same place...

We analysed the presence of nuclear bodies and particularly Cajal bodies during representative stages of gametophytic and haploid embryogenic development in isolated microspore and anther cultures of a model system (Brassica napus cv. Topas) and a recalcitrant species (Capsicum annuum L. var. Yolo Wonder B). The nuclear bodies domain is involved on several important roles on nuclear metabolism,...