In smooth muscle cells (SMCs) isolated from rabbit carotid, femoral, and saphenous arteries, relative myosin isoform mRNA levels were measured in RT-PCR to test for correlations between myosin isoform expression and unloaded shortening velocity. Unloaded shortening velocity and percent smooth muscle myosin heavy chain 2 (SM2) and myosin light chain 17b (MLC(17b)) mRNA levels were not significan...

Transcription of ptsG encoding glucose-specific permease, enzyme IICB(Glc), in Escherichia coli is initiated from two promoters, P1 and P2. ptsG transcription is repressed by Mlc, a glucose-inducible regulator of carbohydrate metabolism. The regulation of ptsG P1 transcription is also under positive control by cyclic AMP receptor protein and cyclic AMP complex (CRP.cAMP) as observed in other Ml...

introduction: multileaf collimator (mlc) is state of the art among the radiation field shaping systems for conformal radiotherapy techniques. some advantages of this system are as follow: reduction of treatment time, higher precision in conforming the radiation field to the projection of the target volume and lowering the chance of errors made by technologists. the purpose of this research was ...

We have previously characterized human smooth muscle myosin light chain (MLC)-2 isoform by complementary DNA cloning and have shown that this isoform is expressed in a number of nonmuscle cells such as fibroblast cells. In this report, we show that when human osteosarcoma derived clonal cells (TE 85 clone F-5) (HOS), which are immortalized and nontumorigenic, undergo transformation following in...

OBJECTIVE Physiological roles of apelin and its specific receptor APJ signaling were investigated in vascular smooth muscle cells (VSMCs). The present study determined whether apelin activates myosin light chain (MLC), a major regulatory event in initiating smooth muscle contraction. METHODS AND RESULTS To assess MLC activation, we performed Western blot and immunohistochemical studies using ...

In cultured porcine aortic endothelial monolayers, the effect of ATP on myosin light chain (MLC) phosphorylation, which controls the endothelial contractile machinery, was studied. ATP (10 microM) reduced MLC phosphorylation but increased cytosolic Ca(2+) concentration ([Ca(2+)](i)). Inhibition of the ATP-evoked [Ca(2+)](i) rise by xestospongin C (10 microM), an inhibitor of the inositol trisph...

ROCK (Rho-kinase), an effector molecule of RhoA, phosphorylates the myosin binding subunit (MBS) of myosin phosphatase and inhibits the phosphatase activity. This inhibition increases phosphorylation of myosin light chain (MLC) of myosin II, which is suggested to induce RhoA-mediated assembly of stress fibers and focal adhesions. ROCK is also known to directly phosphorylate MLC in vitro; howeve...

Introduction Multi-Level Cell (MLC) technology greatly reduces flash die size to achieve a breakthrough cost structure. It does this by storing 2 bits of data per physical cell instead of the traditional 1 bit per cell, using Binary flash technology. But the increased density of the MLC flash media has grave consequences in terms of data reliability and performance. A number of flash vendors, w...

background: the incidence of dentinal defects may infl uence the outcome of root canal treatment. the aims of this study were to evaluate and compare the incidence of dentinal defects following root canal obturation with two different techniques. materials and methods: a total of 110 mesial roots of human mandibular fi rst molars were selected. twenty-seven roots were left unprepared as negativ...