Positioning of reactive functional groups within a DNA duplex can enable chemical reactions that otherwise would not occur to an appreciable extent. However, few studies have quantitatively defined the extent to which the enforced proximity of reaction partners in duplex DNA can favor chemical processes. Here, we measured substantial effective molarities (as high as 25M) afforded by duplex DNA ...

The binding of H1 (and H5) to nucleosome core particles was demonstrated by separating mononucleosomes according to their DNA size on acrylamide gels containing high molarity urea. The presence of urea causes a redistribution of H1 so that it associates with some particles of all linker lengths, including no linker. When the urea is removed the H1 remains associated with particles of all DNA si...

Fibrinogen is quantitatively precipitated from plasma by 1.20 M phosphate buffer. The amount of precipitated fibrinogen is determined turbidimetrically at 450 m5. The method requires 0.25 ml. of plasma for the blank and sample. Reliability of the method has been established by comparison with an ammonium sulfate turbidimetric method and a colorimetric method, demonstration of specificity, varia...

Strains accomodated in the genus Zoophthora are very sensitive to osmotic value of their habitat. Hipertonical molarity of buffers and NaCl decreases the growth, but this effect strongly depends on the species tested and on the kind of buffer. In 0.66% phtalan buffer the growth of Z. lanceolata is completely stopped whereas Z. psyllae and Z. aphrophora is inhibited only in 50% comparing to the ...

The effect of sodium hydroxide (SH) concentration and silicate (SS) content in alkali activator on mechanical properties wet-dry resistance fly ash added pumice based geopolymer paste were investigated. In the study, SH was used as 8, 10, 12 14 molar, while weight ratio SS to 0, 1, 2 3. While density samples increased significantly with increase molarity mixtures low content, it less affected b...

A principal conclusion drawn from observations of time- and concentration-dependent liquid-vapor (LV) interfacial tension gamma(lv) of a diverse selection of proteins ranging from albumin to ubiquitin spanning nearly three decades in molecular weight (MW) is that concentration scaling substantially alters perception of protein interfacial activity as measured by reduction in gamma(lv). Proteins...

This paper presents a novel microwave-assisted hydrothermal technique for synthesizing tin(II) oxide nanoparticles. This technique can be used for producing large quantities of homogeneous nanoparticles in a short time. The effect of the solution molarity, final pH, hydrothermal processing time and microwave power were studied. The tin(II) oxide structure verified from XRD...

A number of methods for the preparation of chick oviduct nuclei have been compared. Nuclei have been isolated in hypertonic sucrose and citric acid and the product has been characterized with respect to cleanliness, ultrastructure, RNA polymerase activity, RNA integrity, and chromatin composition. The study demonstrates that the choice of oviduct nuclear isolation procedure will depend markedly...