Cancer is a leading cause of death worldwide. Despite the availability of several chemotherapeutic drugs, there is still a great need for more efficient agents for a better management of cancer. In this contribution, a series of 11 DHPs (4a, 4b & 7a-i) were synthesized and evaluated for their cytotoxic effect against MCF-7, LS180, and MOLT-4 cancer cell lines using MTT assay. Synthesized 2,6-di...

Terpinen-4-ol is a terpene found in the rhizome of Plai (Zingiber montanum (Koenig) Link ex Dietr.). In this study apoptogenic activity and mechanisms of cell death induced by terpinen-4-ol were investigated in the human leukemic MOLT-4 cell line. Terpinen-4-ol exhibited cytotoxicity in MOLT-4 cells, with characteristic morphological features of apoptosis by Wright's staining. The mode of cell ...

Young pinnipeds, born on land, must eventually enter the water to feed independently. The aim of this study was to examine developmental factors that might influence this transition. The ontogeny of metabolic rate and thermoregulation in northern fur seal, Callorhinus ursinus, pups was investigated at two developmental stages in air and water using open-circuit respirometry. Mean in-air resting...

In search for selective anti-insect agents some monoand bis-isothiocyanates were prepared and tested on third instar larvae of Manduca sexta. The alkylene-bis-isothiocyanates 4, 5 and 6 had a novel effect preventing formation of the abdominal spiracles and of the hooks (crochets) at the following molt to the 4th instar. Some of these larvae died in the molt, while spiracles remained absent also...

We have established that CpG oligodeoxynucleotide 5mers, of sequence type CGNNN (N = A, G, C or T), rapidly induce apoptosis/cell cycle arrest in human leukaemia lines. The 5'-CpG is obligatory for these effects. Induction of apoptosis in MOLT-4 cells did not require new protein synthesis and was insensitive to the caspase 3 inhibitor, Ac-DEVD-CHO, although the latter abrogated DNA laddering, p...

BACKGROUND We have previously demonstrated that phenylhexyl isothiocyanate (PHI), a synthetic isothiocyanate, inhibits histone deacetylases and remodels chromatins to induce growth arrest in HL-60 myeloid leukemia cells in a concentration-dependent manner. METHODS To investigate the effect of PHI, a novel histone deacetylases inhibitor (HDACi), on demethylation and activation of transcription...

background and objective: vascular endothelial growth factor (vegf) is a cytokine which is overexpressed in many malignant cancers including leukemia. vegf plays an important role in tumor invasion and metastasis. determination of the pattern of vegf expression in human leukemic cell lines could be useful not only in screening of new antileukemic agents but also to study the mechanism of their ...

introduction: the tumor suppressor p53 protein can induce apoptosis in some cellular contexts. among the apoptosis-inducing genes, p53 has received the most attention for cancer gene therapy. in this study, the role of dendrosome and lipofectin mediated normal cdna of tp53 into molt-4, ccrf-cem(t-iymphoma) and k562 cell lines was assessed. material and methods: at first, ccrf-cem, mol t-4 and k...

Molting is a natural process, which birds undergo to rejuvenate their reproductive organs. The US poultry egg production industry has used feed withdrawal to effectively induce molt; however, susceptibility of Salmonella Enteritidis has encouraged the development of alternative methods. Previous research conducted in our laboratory showed that alfalfa is effective at molt induction and provides...