Methods were studied for the cryopreservation of microfilariae of periodic Brugia malayi. RPMI-1640 tissue culture medium containing 6% dimethyl sulfoxide (DMSO) and 15% newborn calf serum was used as cryoprotectant. Samples were frozen slowly in the vapor phase of liquid nitrogen prior to emersion in liquid nitrogen (-196 degrees C). The freezing rate was -0.5 to -1.0 degrees C per minute, mic...

Foi desenvolvida a armadilha ventilada como proposta de novo método para coleta mosquitos com presença uma pessoa no interior dela, que serve atrativo, mas permanece protegida das picadas dos mosquitos, empurrados um coletor (onde ficam aprisionados) pela ação ventiladores. Uma variação desse aparato também foi apresentada utilização atrativos químicos, gás carbônico (CO2), octenol (1-octen-3-o...

for over a year ago, but Lieutenant-Colonel Giles found that so many changes were necessary, and so much new knowledge had to be added that nothing less than completely re-writing his book would be of any avail. We have no reason to regret the inevitable delay : the present volume has grown to over 520 pages instead of 370, and in every respect the new volume is an improvement on the older one....

4. Myzomyia Rossi (Giles). Very common.12 5. Cellia pulcherrima. Very common.4 6. Mysomyia Turkhudi (Liston). Three specimens only 14 7. Myzorhynchus sinensis (Wied). Three specimens only 3 8. Nyssorhynchus Jamesi (Theobald). Very scarce; three specimens." 9. Nyssorhynchus Maculipalpis. One specimen.7 10. Myzorhynchus barbirostris (Wulp). Larva only found/2 (There are three more species in my c...

background: the intracellular protozoan parasites of the genus haemoproteus occur in different avian hosts all over the world. various genus of blood sucking insects’ families such as hippoboscidae and ceratopogonidae could transmit haemoproteus in avian hosts. there are very limited number of studies on wild infected birds with blood parasites in iran, so the aim of this study was to determine...

Los Flavivirus constituyen virus transmitidos por artrópodos, principalmente mosquitos. Pueden producir enfermedades en humanos y animales, también incluyen específicos de insectos que solo infectan se replican los insectos, no así vertebrados. En Paraguay dengue, fiebre amarilla Zika fueron detectados infecciones humanas, pero estudios flavivirus mosquitos son aún escasos. Por ello, el objetiv...

A study was made of the oviposit behavior of gravid female Aedes mosquitos in man-made habitats under field conditions. The study showed that the gravid female Aedes mosquitos preferred containers with relatively easy access but not too open to external environmental influence. The dark surface of the containers served as the initial and long-range attractant to the breeding sites. Volatile che...