rate of in vitro maturation of oocytes is one of the challenges of assisted reproductive techniques. in this study we investigated the effects of supplementation of cysteamine on the rate of in vitro maturation of oocytes in two different media. germinal vesicle oocytes were collected from mouse ovary and cultured in two media (tcm199 and meme) with 0, 50, 100, 200, 500 µm/ml cysteamine. number...

background:   objective: this study was designed to examine the effects of hp on in-vitro maturation of oocytes and cell death in cumulus cells derived from vitrified-warmed mouse ovaries.   materials and methods: preovulatory follicles were harvested from non-vitrified and vitrified-warmed 6-8 week-old female nmri mouse ovaries and randomly assigned to following groups: non-vitrified (control)...

Objective Oocyte cryopreservation is an essential part of assisted reproductive technology (ART) which was recently introduced into clinical practice. This study aimed to evaluate the effects of two vitrification systems (Cryotop and Open Pulled Straw, OPS) on mature oocytes gene expressions. MaterialsAndMethods The survival rate of metaphase II (MII) mouse oocytes were assessed after cryoprese...

Background: It is important to protect oocytes and embryos from oxidative stress in the culture medium. Melatonin has been shown to be a direct free radical scavenger. Therefore, effect of melatonin during in vitro oocyte maturation (IVM), fertilization and embryo development of mouse oocytes was evaluated. Materials and Methods: Oocytes from supper-ovulated mouse were divided to two groups: cu...

Background: In vitro culture of ovarian follicles may preserve fertility in women with premature ovarian failure due to cancer .It seems that creation a condition that could maintain cellular communications and supports growth of follicles to produce mature oocytes appear to be essential. Nitric oxide (NO) has been recently shown to act with a dual action in mouse oocyte meiotic maturation depe...

Background and aims: Various culture media along with some supplements and enrichment materials have been suggested for in vitro maturation. This study aims to investigate the effect of retinoic acid on nuclear maturation of immature mouse oocytes in vitro. Materials and methods: immature oocytes were obtained from 6-8 weeks old NMRI mice, 48 hours after intraperitoneal injection of 7.5 units ...

Mice are ideal models for ICSI experiments because of the ease of culturing eggs/embryos in vitro and the availability of ample genetic information. Unfortunately, it has been extremely difficult. In this study we improved the mouse ICSI method by using a new holding pipette that was made of two pipettes such that one pipette was pulled and heat merged into the other one. The outer pipette ...

BACKGROUND Mouse oocytes can be used in conjunction with intracytoplasmic sperm injection (ICSI) as a technique to permit chromosomal analysis of human sperm. However, chromosomes derived both from the human sperm and the mouse oocyte appear simultaneously following ICSI. The present study focused on evaluating whether or not previously enucleated mouse oocytes are usable for the analysis of hu...