The bile tests for characterizing gram-negative anaerobic bacilli were reevaluated in prereduced anaerobically sterilized peptone-yeast-glucose broth, in thioglycollate broth, and on blood agar plates. Blood agar plates were unsatisfactory. The combination of 20% bile with 0.1% desoxycholate inhibited Fusobacterium, Bacteroides melaninogenicus, and B. oralis and sometimes Sphaerophorus necropho...

Background: In children, caries removal by means of conventional rotary instruments is often associated with pain and discomfort. An alternative method to conventional caries removal by means of rotary instruments is the use of chemo-mechanical caries removal agents. Aim: To evaluate and compare the efficacy of two chemomechanical caries removal agents Carisolv and Papacarie in reducing carioge...

INTRODUCTION: Phosphatidylcholine (PC) is a predominant phospholipid present in bile protecting bile ducts from harmful effects of bile acids. In our earlier studies on the analysis bile from cholestatic patients using H MRS, we had observed the hydrolysis of phosphatidylcholine in some patients [1]. Since phosphatidylcholine and its hydrolysis products – glycerophosphocholine (GPC), and phosph...

Introduction: Probiotics are nonpathogen living microorganisms which express beneficial effects on host, when are administered in adequate amounts. Traditional dairy products are regarded as good sources of probiotics. Present study aims to isolate Lactic Acid Bacteria (LAB) from Siahmezgi cheese, a traditional cheese produced in the north of Iran, and to evaluate their probiotic potential for ...

INTRODUCTION: Bile acids, phospholipids, and cholesterol are the major lipid components in human bile [1]. The composition of bile is altered in various chronic cholestatic diseases and determining such alterations will be of great clinical importance in understanding the pathophysiology of these diseases [2]. Although there are a few chromatographic [3] and NMR-based methods [4,5] available fo...

Colistin-polymyxin B-cellobiose agar was employed for the isolation of Vibrio vulnificus from shellfish. Isolates were examined phenotypically and with a gene probe and monoclonal antibody specific for V. vulnificus. Results indicated that colistin-polymyxin B-cellobiose agar is superior to both sodium dodecyl sulfate-polymyxin B-sucrose agar and thiosulfate-citrate-bile salts-sucrose agar in i...

A new selective and differential agar medium, polymyxin-mannose-tellurite (PMT) agar was devised to differentiate easily colonies of Vibrio cholerae O1 from those of V. cholerae non-O1. The differentiation between colonies of the two vibrios is based on mannose-fermentation. Colonies of V. cholerae O1 on the agar are agglutinated with O1 antiserum of V. cholerae much more easily than those on t...

Background: The pickle, a traditional fermented product, is popular among Iranians. Much research has been conducted worldwide on this food group. Due to a lack of related data in Iran, this study was conducted to isolate and identify dominant lactic acid bacteria (LAB) in pickles and salted pickles. Materials and methods: Seventy samples were collected from different regions of Iran. The isola...