Somatic embryogenesis was initiated in C. sativus L. from shoot meristems on LS medium containing BAP (2×10-5 M)+NAA (2×10-5 M). Various stages of somatic embryogenesis were observed in the same medium and the development was asynchronous. Somatic embryo development proceeded through well recognized sequences (globular to embryoids) with clearly discernible bipolar regions. Matured embryos coul...

An efficient protocol for callus induction and somatic embryogenesis of Cyclamen premium Mill. Was developed. In this study we investigate difference between response of two explants type leaf and petiole and two medium salt concentrations MS and 1⁄2 MS on callus percentage and somatic embryo number. Media was non-significant on callus production but leaf explants was better than petiole and in...

An efficient in vitro protocol was standardized for Almond (Prunus dulcis) propagation using dormant axillary buds as explants. Explants were cultured on Murashige and Skoog (MS) and woody plant medium (WPM) supplemented with different concentration/combination(s) of phytohormones. MS basal medium showed lowest shoot induction and took longest duration for shoot initiation. Multiple shoots were...

Matthiola incana (Brassicaceae) is an important ornamental plant, which is used as cut flower, pot plant, and landscape. Traditionally, it is propagated via seeds, but interest is given in vegetative propagation of parental lines as well as superior single plants. Micropropagation by organogenesis is an efficient in vitro propagation method for Matthiola incana. Starting from seeds, apical bud ...

An experiment was conducted to study the effect of growth regulators on callus induction in rice embryo culture. In this experiment, caullogenesis was initiated from the matured seeds of seven indica rice varieties viz., TRY 1, TRY 2, Pokkali, CSR 10, W.Ponni, BPT 5204 and IR 29. The medium used for this callus induction was Murashige and Skoog (1962) commonly referred as MS medium with six dif...

background and objectives: bitter melon is one of the anti-cancer medicinal plants that it’s wide level in vitro propagation and anti-cancer material production was necessary. different factors such as genotype, selected explants’ type and it`s preparation conditions influenced regeneration content. plant growth regulator`s effect on callogenesis and indirect regeneration from parangipetai indi...

Development of highly efficient and reproducible plant regeneration system has tremendous potential to provide improved technology to assist in genetic transformation of indica rice cultivars for their further exploitation in selection. For the development of a highly reproducible regeneration system through somatic embryogenesis, mature embryos of highly popular rice cultivars i.e., Govind (fo...

The effects of plant growth regulators were examined in order to optimize the callus induction, regeneration, and proliferation of lisianthus (Eustoma grandiflorum). In vitro leaves provided the explants for callus induction. Explants were cultured on Murashige and Skoog (MS) medium with different concentrations of indole-3-acetic acid (IAA), 1-naphthaleneacetic acid (NAA), and 2,4-dichlorophen...

In vitro studies were carried out with Ochradenus arabicus for rapid micropropagation from embryo explants. Direct regeneration of shoot buds of O. arabicus was observed in MS basal medium supplemented with varying levels of either benzyladenine (BA) or kinetin (Kn). Embryos produced multiple shoots on Murashige and Skoog’s medium (MS) containing 1.0 μM BA after 25 days of inoculation. Number o...

Rhizomes of Smilax corbularia are used for the treatment of cancers, AIDS, septicemia and lymphatic diseases. The demand for rhizomes as an important ingredient in medicinal preparations has increased considerably. As rapid multiplication through in vitro propagation need to be developed, the effects of MS (Murashige and Skoog) medium salt strengths and plant growth regulators were investigated...