Tuberculosis is a chronic disease caused by Mycobacterium tuberculosis (Mtb), and many previous studies indicated that Hsp16.3 may play an important role in long-term survival of Mtb within macrophages. However, the underlying mechanism of Hsp16.3 in protecting of Mtb is not clear. In this study, we constructed a prokaryotic expression vector, pET28a-Mtb Hsp16.3, which induced the expression of...

ObjectiveTo evaluate he diagnostic performance of the Xpert Mycobacterium tuberculosis/Rifampin (MTB/RIF) assay in bronchoalveolar lavage fluid (BALF).MethodsWe retrospectively reviewed clinical data from 671 sputum-smear negative or sputum-scarce adult patients with suspected pulmonary tuberculosis (PTB) who had an MTB/RIF performed on BALF. The assay, smear microscopy (SM) and MTB culture was...

To survive and replicate in macrophages Mycobacterium tuberculosis (Mtb) has developed strategies to subvert host defence mechanisms, including autophagy. Autophagy induction has the potential to clear Mtb, but little is known about its effect during controlled tuberculosis and HIV co-infection. Mammalian target of rapamycin complex1 (mTORC1) inhibitors were used to induce autophagy in human ma...

Background: Tuberculosis (TB) diagnostics, such as Xpert MTB/RIF, is still limited by cost. Testing of pooled samples from presumptive TB patients has been thought a cost-saving strategy to diagnose TB. We assessed the utility and MTB/RIF testing for diagnosis in Mwanza, Tanzania. Methods: Sputum Presumptive were submitted laboratory routine using MTB/RIF. The results individual sputum used ref...

Purpose: To develop a novel detection technology for drug-resistance mutation sites of Mycobacterium tuberculosis (MTB) using Luminex liquid chip.Methods: Using polymerase chain reaction (PCR) amplification and hybridization analysis, MTB infection drug-resistant the first-line second-line anti-MTB drugs were simultaneously identified. A method was applied to analyze wild-type standard strains ...

The severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) and Mycobacterium tuberculosis (Mtb) coinfection has been observed in a number of nations it is connected with illness death. paper studies reaction–diffusion within-host Mtb/SARS-CoV-2 model immunity. This explores the connections between uninfected epithelial cells, latently Mtb-infected productively SARS-CoV-2-infected free Mtb...

A previous unbiased genome-wide analysis of CD4 Mycobacterium tuberculosis (MTB) recognition using peripheral blood mononuclear cells from individuals with latent MTB infection (LTBI) or nonexposed healthy controls (HCs) revealed that certain MTB sequences were unexpectedly recognized by HCs. In the present study, it was found that, based on their pattern of reactivity, epitopes could be divide...

BACKGROUND Previous studies have shown that Mycobacterium tuberculosis (MTB) Uganda family, a sub-lineage of the MTB Lineage 4, is the main cause of tuberculosis (TB) in Uganda. Using a well characterized patient population, this study sought to determine whether there are clinical and patient characteristics associated with the success of the MTB Uganda family in Kampala. METHODS A total of ...

OBJECTIVE Understanding human immunity to Mycobacterium tuberculosis (Mtb) during different stages of infection is important for development of an effective tuberculosis (TB) vaccine. We aimed to evaluate immunity to Mtb infection by measuring immune responses to selected Mtb antigens expressed during different stages of infection over time and to observe sustainability of immunity. METHODS I...

Defining the mechanisms of Mycobacterium tuberculosis (Mtb) persistence in the host macrophage and identifying mycobacterial factors responsible for it are keys to better understand tuberculosis pathogenesis. The emerging picture from ongoing studies of macrophage deactivation by Mtb suggests that ingested bacilli secrete various virulence determinants that alter phagosome biogenesis, leading t...