Corynebacterium diphtheriae, Corynebacterium ulcerans and Corynebacterium pseudotuberculosis constitute a group of potentially toxigenic microorganisms that are related to different infectious processes in animal and human hosts. Currently, there is a lack of information on the prevalence of disease caused by these pathogens, which is partially due to a reduction in the frequency of routine lab...

background escherichia coli o157:h7, vibrio cholerae, and salmonella typhimurium are pathogenic bacteria found in contaminated water and food. no assay method is currently available on simultaneous detection or identification of all the three pathogens. our aim was to develop a rapid and reliable method for this purpose. materials and methods a protocol for sample collection, and a pcr procedur...

BACKGROUND & OBJECTIVES Bacillus anthracis, Yersinia pestis, Burkholderia pseudomallei and Brucella species are potential biowarfare agents. Classical bacteriological methods for their identification are cumbersome, time consuming and of potential risk to the handler. METHODS We describe a sensitive and specific multiplex polymerase chain reaction (mPCR) assay involving novel primers sets for...

AIM This study was aimed to detect Mannheimia haemolytica in lung tissues of sheep and from a bacterial culture. INTRODUCTION M. haemolytica is one of the most important and well-established etiological agents of pneumonia in sheep and other ruminants throughout the world. Accurate diagnosis of M. haemolytica primarily relies on bacteriological examination, biochemical characteristics and, bi...

Campylobacteriosis caused by Campylobacter spp. is the prime cause of bacterial gastroenteritis worldwide. Chicken and other poultry birds act as major reservoir for thereby play a crucial role in transmission this zoonotic disease to humans. The current investigation was undertaken study occurrence organised layer farms their associated environmental samples. A total 260 samples comprising clo...

conclusions this is the first report on the prevalence of iron acquisition genes among a. baumannii isolates collected from patients with urinary tract infections. the high prevalence of iron acquisition genes in a. baumannii isolates suggests that these virulence factors play an important role in the development of urinary tract infections. results a high prevalence of genes encoding iron acqu...

OBJECTIVE To evaluate multiplex allele specific polymerase chain reaction as a rapid molecular tool for detecting multidrug-resistant tuberculosis. METHODS Based on drug susceptibility testing, 103 isolates were multidrug-resistant tuberculosis and 45 isolates were sensitive to isonicotinylhydrazine and rifampin. Primers were designed to target five mutations hotspots that confer resistance t...

A rapid method for detection of Escherichia coli O157: H7 using multiplex PCR was developed. Two oligonucleotide primer pairs were used for simultaneously detection of vt encoding verotoxin genes for virulence factor and rfbO157 encoding the O-antigen specific for E. coli O157: H7. Multiplex PCR generated two products of 215 bp and 420 bp for vt and rfbO157, respectively. Multiplex PCR detected...