A novel nonconjugative plasmid of 28,489 bp from a porcine linezolid-resistant Enterococcus faecium isolate was completely sequenced. This plasmid harbored a novel type of multiresistance gene cluster that comprised the resistance genes lnu(B), lsa(E), spw, aadE, aphA3, and two copies of erm(B), which account for resistance to macrolides, lincosamides, streptogramins, pleuromutilins, streptomyc...

Transcriptional organization of bla(CTX-M-2) present in a multiresistance plasmid of Salmonella enterica serovar Infantis suggests the presence of more than one promoter involved in the expression of the beta-lactamase gene. At least two bla(CTX-M-2)-specific mRNAs (near to 1 kb and 5 kb) were evidenced. Two +1 signals were detected at -22 bp and -59 bp of bla(CTX-M-2) defining two putative pro...

Current concerns about multiresistance and a diminishing antibiotic pipeline are mainly addressed to Gram-negative bacteria. The greatest fear within the Gram-positive arena is vancomycin-resistant Staphylococcus aureus. Its epidemiology and clinical presentation give cause for concern, but so far its impact has been strictly limited. While this may change, the loss of glycopeptides as a treatm...

The majority of Salmonella strains isolated in veterinary laboratories in Poland in 2001 originated from poultry (82%), swine (4%), and animal feeding stuffs (10%). Thirty-two serovars were observed among tested strains. Antimicrobial susceptibility testing revealed the recent trends in Salmonella resistance. The increase in the number of resistant strains was observed. Multiresistance occurred...

ABSTRACT The emergence of livestock-associated methicillin-resistant Staphylococcus aureus strains (LA-MRSA) and the potential role pigs in evolution these has led to increased interest research microorganisms. However, this contributed a lack isolation characterization methicillin-susceptible S. (MSSA). In study, prevalence nursery finishing stages were analyzed. susceptibility profiles antibi...

OBJECTIVES The multiresistance plasmid pKP96 from Klebsiella pneumoniae was sequenced completely and analysed concerning its genetic environment and distributing of antimicrobial resistance genes. METHODS The complete sequence of the plasmid was determined using a whole-genome shotgun approach. MICs of 13 antimicrobial agents were determined using Etests. A conjugation experiment was performe...

OBJECTIVES To investigate the prevalence and genetic environment of the multiresistance gene oqxAB in Salmonella enterica serotype Typhimurium isolated from food-producing animals. METHODS In this study, 63 Salmonella enterica serotype Typhimurium isolates were analysed for the presence of plasmid-mediated quinolone resistance determinants and mutations in the quinolone resistance-determining...

The multiresistance plasmid pJHCMW1, harbored by a clinical Klebsiella pneumoniae strain isolated from a neonate with meningitis, was sequenced. A circular sequence of 11,354 bp was generated, of which 7,993 bp make up Tn1331, a transposon including the antibiotic resistance genes aac(6')-Ib, aadA1, bla(OXA-9), and bla(TEM-1). The gene aac(6')-Ib is included in a gene cassette, and both aadA1 a...