BACKGROUND This study describes the ultrastructure of long-term denervated rat extensor digitorum longus and tibialis anterior muscles, with particular emphasis on understanding the cellular basis for the reduced restorative capacity of long-term denervated muscle. METHODS In 30 male WI/HicksCar rats, the right hindleg was denervated for periods of 1, 2, 4, 5.5, 6, 7, 12, 14, and 18 months be...

Previous measurements of muscle thickness and length ratio of costal diaphragm insertions in the dog (A. M. Boriek and J. R. Rodarte. J. Appl. Physiol. 77: 2065-2070, 1994) suggested, but did not prove, discontinuous muscle fiber architecture. We examined diaphragmatic muscle fiber architecture using morphological and histochemical methods. In 15 mongrel dogs, transverse sections along the leng...

this study was conducted on 48 specimens of rectus abdominis muscles for recognition and definition of passage and ramification of lower intercostal nerves. the main results of this study are as follows: 1- the seventh and eight intercostal nerves penetrated to posterior layer of the rectus sheath while other intercostal nerves perforated to dorsal layer of internal oblique abdominis aponeurosi...

muscle biopsy interpretation has been revolutionized by ihc. immunohistochemistry now has an essential role in the evaluation of the muscle biopsies and in examining proteins localizations. advances in the characterization of sarcolemmal proteins and recognition that defects in the genes encoding such proteins may lie at the heart of the multiple differing forms of muscular dystrophy have been ...

In the present study, the lenses and ciliary bodies of 20 ostrich eyes were studied macroscopically and microscopically. The histological slides were studied after staining by hematoxylin and eosin, Verhoeff, Van Gieson, and periodic acid-Schiff (PAS). Posterior surface of lens was more convex than its anterior surface. The average lens diameter and thickness were respectively measured as 1.43 ...

The cellular localization of the peptide neurotransmitter proctolin was determined for larvae of the fruitfly Drosophila melanogaster. Proctolin was recovered from the CNS, hindgut, and segmental bodywall using reverse-phase HPLC, and characterized by bioassay, immunoassay, and enzymatic analysis. A small, stereotyped population of proctolin-immunoreactive neurons was found in the larval CNS. S...

The muscle fiber composition and cross-sectional area of muscle fiber types were investigated histochemically in the abdominal muscles (rectus abdominis muscle, obliquus externus abdominis muscle, obliquus internus abdominis muscle and transversus abdominis muscle) of three Japanese macaques (Macaca fuscata). Muscle fibers were classified into three fiber types (Type I, II A and II B) by myosin...

Patients with pulmonary hypertension (PH) suffer from inspiratory muscle weakness. However, the pathophysiology of inspiratory muscle dysfunction in PH is unknown. We hypothesized that weakness of the diaphragm, the main inspiratory muscle, is an important contributor to inspiratory muscle dysfunction in PH patients. Our objective was to combine ex vivo diaphragm muscle fiber contractility meas...

When muscle fibers contract, blood flow requirements increase along their entire length. However, the organization of capillary perfusion along muscle fibers is unclear. The microvascular unit (MVU) is defined as a terminal arteriole and the group of capillaries it supplies. We investigated whether neighboring MVUs along the fiber axis perfused the same group of muscle fibers by using the paral...