Grey seal skeletal muscle containing high levels of myoglobin was used to prepare poly(A)+ RNA. In vitro translation of this RNA produced a range of polypeptides including myoglobin. cDNA was prepared by reverse transcription of muscle poly(A)+ RNA and cloned into the plasmid pAT 153. 4% of cDNA recombinants were shown to contain myoglobin cDNA inserts. DNA sequence analysis of one clone (pSM 1...

HE characteristics of myoglobin ifz citro have been demonstrated to be those of a respiratory pigment, but the role of myoglobin ilz vivo has yet to be fully established. The literature to date affords two substantiated concepts with regard to its physiologic muscle as a distinct heme pigment, nature; namely, and that it may that myoglobin exists in striated act as a short-time oxygen store in ...

Tryptic and chymotryptic digests of human myoglobin have been separated chromatographically to give 23 pure tryptic peptides and 18 pure chymotryptic peptides. The complete or partial amino acid sequences of these peptides have been established. From the overlaps in sequence between chymotryptic and tryptic peptides it is possible to place 124 of the 152 residues of human myoglobin in a unique ...

ANTHONY,ADAM,EUGENEACKERMAN AND G.K.STROTHER. Effects of altitude acclimatization on rat myoglobin. Changes in myoglobin content of skeletal and cardiac muscle. Am. J. Physiol. 196(s): 512-516. 1959. -Analyses were made of myoglobin content of rat skeletal and cardiac muscle following continuous exposure to simulated altitudes of 18,000 feet for a Q--IO-week period. About five dozen rats were u...

Tryptic and chymotryptic digests of human myoglobin have been separated chromatographically to give 23 pure tryptic peptides and 18 pure chymotryptic peptides. The complete or partial amino acid sequences of these peptides have been established. From the overlaps in sequence between chymotryptic and tryptic peptides it is possible to place 124 of the 152 residues of human myoglobin in a unique ...

ANTHONY,ADAM,EUGENEACKERMAN AND G.K.STROTHER. Effects of altitude acclimatization on rat myoglobin. Changes in myoglobin content of skeletal and cardiac muscle. Am. J. Physiol. 196(s): 512-516. 1959. -Analyses were made of myoglobin content of rat skeletal and cardiac muscle following continuous exposure to simulated altitudes of 18,000 feet for a Q--IO-week period. About five dozen rats were u...

We compared the clinical usefulness of serum myoglobin and creatine kinase MB (CK MB) isoenzyme determinations in the early diagnosis of acute myocardial infarction in 109 consecutive patients admitted to a coronary care unit. Of these, 37 patients were diagnosed as having definite infarction, three possible infarction, and 69 no infarction, using World Heath Organisation criteria. Blood sample...

An evaluation of porcine longissimus myoglobin concentration was conducted to determine breed and gender differences for myoglobin content, estimate genetic parameters for myoglobin concentration, and determine the relationship between myoglobin content and objective measures of muscle color. Data from centrally tested (n = 255), purebred Yorkshire (42), Duroc (61), Hampshire (17), Chester Whit...

The aim of this prospective study was to evaluate and compare the relative increase of serum myoglobin level and total creatine kinase (CK) activity in acute myocardial infarction (AMI) patients (n=36). We measured serial changes in total CK activity and myoglobin serum level in three-time periods (6-9 hours, 24 hours and 6-7 days) from chest pains onset. Myoglobin peaked during the first 6-9 h...

introduction: ursolic acid (ua) is a lipophilic triterpenoid compound, found in large amounts in apple peel. anabolic effects of ua on the skeletal muscle and the role of this tissue as a key regulator of systematic aging aroused this question in mind whether ua might amend skeletal muscle performances such as myoglobin expression and also whether it switches skeletal muscle fibers from glycoly...