DISEASE resistance gene cloning is one of the main targets in modern sugarcane research. In this study, several primers were designed according to the conserved motifs in the NBS regions of known resistance genes to amplify sequences from cDNA of sugarcane variety NCo376 by polymerase chain reaction (PCR). In total, six NBS-LRR type resistance gene analogs (RGAs) were cloned, for which the dedu...

Nucleotide binding site leucine-rich repeats (NBS-LRR) disease resistance proteins play an important role in plant defense against pathogen attack. A number of recent studies have been carried out to identify and characterize NBS-LRR gene families in many important plant species. In this study, we identified NBS-LRR gene family comprising of 1015 NBS-LRRs using highly stringent computational me...

Disease resistance in plants is often controlled by a gene-for-gene mechanism in which avirulence (avr) gene products encoded by pathogens are specifically recognized, either directly or indirectly, by plant disease resistance (R) gene products. Members of the NBS-LRR class of R genes encode proteins containing a putative nucleotide binding site (NBS) and carboxyl-terminal leucine-rich repeats ...

The nucleotide-binding site (NBS)-Leucine-rich repeat (LRR) gene family accounts for the largest number of known disease resistance genes, and is one of the largest gene families in plant genomes. We have identified 333 nonredundant NBS-LRRs in the current Medicago truncatula draft genome (Mt1.0), likely representing 400 to 500 NBS-LRRs in the full genome, or roughly 3 times the number present ...

Plant disease resistance genes have been shown to be subject to positive selection, particularly in the leucine rich repeat (LRR) region that may determine resistance specificity. We performed a genome-wide analysis of positive selection in members of the nucleotide binding site (NBS)-LRR gene family of Arabidopsis thaliana. Analyses were possible for 103 of 163 NBS-LRR nucleotide sequences in ...

Most known plant disease-resistance genes (R genes) include in their encoded products domains such as a nucleotide-binding site (NBS) or leucine-rich repeats (LRRs). Sequences with unknown function, but encoding these conserved domains, have been defined as resistance gene analogues (RGAs). The conserved motifs within plant NBS domains make it possible to use degenerate primers and PCR to isola...

Comparative study of disease resistance genes in crop plants and their relatives provides insight on resistance gene function, evolution and diversity. Here, we studied the allelic diversity of the Lr10 leaf rust resistance gene, a CC-NBS-LRR coding gene originally isolated from hexaploid wheat, in 20 diploid and tetraploid wheat lines. Besides a gene in the tetraploid wheat variety 'Altar' tha...

| 5 Abstract Developing high yielding and high quality varieties with broad-spectrum and durable disease resistance is the ultimate goal of crop breeding. Resistance (R) genes encoding proteins with nucleotide-binding site (NBS) and leucine-rich repeat (LRR) domains provide high levels of resistance, but this type of R genes usually only confers resistance to a subset of pathogen races. In addi...

Plant resistance ( R ) genes encode proteins that mediate the recognition of corresponding pathogen-encoded avirulence (Avr) proteins, triggering localized cell death (the hypersensitive response) and systemic acquired resistance. A large number of R genes have been characterized from numerous plant species that collectively confer resistance to a wide range of pathogens, including viral, bacte...

The tobacco N gene is a member of the Toll-interleukin-1 receptor/nucleotide-binding site/leucine-rich repeat (TIR-NBS-LRR) class of plant resistance (R) genes and confers resistance to tobacco mosaic virus (TMV). We investigated the importance of specific domains of N in inducing TMV resistance, by examining various N deletion and point mutations that introduce single amino acid substitution m...