To study the stage of development of oxidase activity responsible for the respiratory burst in human granulocytes. we investigated the effect of phorbol myristate acetate (PMA) and fluoride (F ) on nitroblue tetrazolium (NBT) reduction by bone marrow and circulating granulocytic cells in 1 3 subjects without leukocyte functional disorders and a patient with chronic granulomatous disease (CGD) ....

The mechanisms by which bone resorbing osteoclasts form and are activated by hormones are poorly understood. We show here that the generation of oxygen-derived free radicals in cultured bone is associated with the formation of new osteoclasts and enhanced bone resorption, identical to the effects seen when bones are treated with hormones such as parathyroid hormone (PTH) and interleukin 1 (IL-1...

A specific configuration of the global subduction system is parallel triple subduction. The widely accepted example New Guinea region, including a northward dip at Britain Trench (NBT), southward Trobriand Trough (TT), and North Solomon (NST). Questions regarding remain largely unexplored in terms factors controlling its initiation, duration, dynamics. Here, we used two-dimensional numerical mo...

Numerous evolutionary studies have sought to explain the distribution of diversity across the limbs of the tree of life. At the same time, ecological studies have sought to explain differences in diversity and relative abundance within and among ecological communities. Traditionally, these patterns have been considered separately, but models that consider processes operating at the level of ind...

Dear Editor, The O2-generating enzyme NADPH oxidase, plays a crucial role in host defense against microbial infection through the production of reactive oxygen species (ROS).1The multisubunit NADPH oxidase complex can be detected in vitro by the nitroblue tetrazolium test (NBT).2The NBT test is used for the diagnosis of chronic granulomatous disease.3 However, several factors, such as some cyto...

BACKGROUND To determine glycoserum, the nitroblue tetrazolium (NBT) assay is quick, economical, convenient and easily automated. This method, however, is vulnerable to interference by thiol group, which should not be ignored during the analysis. METHODS Thiol group in glycoserum was blocked with iodoacetamide (IAM) before NBT was added. The reaction was carried out in a thermal bath and termi...

Normal human granulocytes are able to reduce the yellow dye, nitroblue tetrazolium (NBT), to insoluble blue formazan. This has been a useful reaction for the recognition of chronic granulomatous disease of childhood (CGD) and the carrier state, because granulocytes froin these patients are deficient in their ability to reduce this dye(l). It has also been suggested that granulocytes from patien...

Human T cell lymphotropic Virus type-1 (HTLV-1) induces lymphocyte activation and proliferation, but little is known about the innate immune response due to HTLV-1 infection. We evaluated the percentage of neutrophils that metabolize Nitroblue tetrazolium (NBT) to formazan in HTLV-1 infected subjects and the association between neutrophil activation and IFN-gamma and TNF-alpha levels. Blood was...

The feeling of error (FOE) is the subjective experience that something went wrong during a reasoning or calculation task. The main goal of the present study was to assess the accuracy of the FOE in the context of mental mathematical calculation. We used the number bisection task (NBT) to evoke this metacognitive feeling and assessed it by asking participants if they felt they have committed an ...