The proteins NCX1, NCX2, and NCX3 expressed on the plasma membrane of neurons play a crucial role in ionic regulation because they are the major bidirectional system promoting the efflux and influx of Na and Ca ions. Here, we demonstrate that NCX1 and NCX3 proteins are novel additional targets for the survival action of the phosphatidylinositol 3-kinase (PI3-K)/ Akt pathway. Indeed, the doxycyc...

The Na(+)-Ca2+ exchanger from Drosophila was expressed in Xenopus and characterized electrophysiologically using the giant excised patch technique. This protein, termed Calx, shares 49% amino acid identity to the canine cardiac Na(+)-Ca2+ exchanger, NCX1. Calx exhibits properties similar to previously characterized Na(+)-Ca2+ exchangers including intracellular Na+ affinities, current-voltage re...

BACKGROUND Noxious stimulation and nerve injury induce an increase in intracellular Ca(2+) concentration ([Ca(2+)]i) via various receptors or ionic channels. While an increase in [Ca(2+)]i excites neurons, [Ca(2+)]i overload elicits cytotoxicity, resulting in cell death. Intracellular Ca(2+) is essential for many signal transduction mechanisms, and its level is precisely regulated by the Ca(2+)...

Nicholas, Susanne B., and Kenneth D. Philipson. Cardiac expression of the Na1/Ca21 exchanger NCX1 is GATA factor dependent. Am. J. Physiol. 277 (Heart Circ. Physiol. 46): H324–H330, 1999.—The cardiac sarcolemmal Na1/Ca21 exchanger plays a primary role in Ca21 efflux and is important in regulating intracellular Ca21 and beat-to-beat contractility. Of the three Na1/Ca21 exchanger genes cloned (NC...

We investigated the properties and interaction domains of SN-6 [2-[4-(4-nitrobenzyloxy)benzyl]thiazolidine-4-carboxylic acid ethyl ester], a newly synthesized and selective Na /Ca exchange (NCX) inhibitor. SN-6 (0.3–30 M) inhibited preferentially intracellular Na -dependent Ca uptake (i.e., the reverse mode) compared with extracellular Na -dependent Ca efflux (i.e., the forward mode) in NCX1-tr...

We investigated the pharmacological properties and interaction domains of N-(3-aminobenzyl)-6-{4-[(3-fluorobenzyl)oxy]phenoxy} nicotinamide (YM-244769), a novel potent Na(+)/Ca(2+) exchange (NCX) inhibitor, using various NCX-transfectants and neuronal and renal cell lines. YM-244769 preferentially inhibited intracellular Na(+)-dependent (45)Ca(2+) uptake via NCX3 (IC(50) = 18 nM); the inhibitio...

Calcium export is a key function for the enamel organ during all stages of amelogenesis. Expression of a number of ATPase calcium transporting, plasma membrane genes (ATP2B1-4/PMCA1-4), solute carrier SLC8A genes (sodium/calcium exchanger or NCX1-3), and SLC24A gene family members (sodium/potassium/calcium exchanger or NCKX1-6) have been investigated in the developing enamel organ in earlier st...

The proteins NCX1, NCX2, and NCX3 expressed on the plasma membrane of neurons play a crucial role in ionic regulation because they are the major bidirectional system promoting the efflux and influx of Na(+) and Ca(2+) ions. Here, we demonstrate that NCX1 and NCX3 proteins are novel additional targets for the survival action of the phosphatidylinositol 3-kinase (PI3-K)/Akt pathway. Indeed, the d...

The importance of Na+/Ca2+ exchangers in the regulation of the physiological and pathological functions of the nervous system has been widely recognized. In this study, we used primary cultured E14.5 cortical neurons as a model system to study the possible roles of the reverse mode Na+/Ca2+ exchange activity in neurotransmission. Using RT-PCR, several exchanger isoforms, ncx1, ncx3 and nckx2-4 ...