The large-scale production of cardiomyocytes is a key step in the development of cell therapy and tissue engineering to treat cardiovascular diseases, particularly those caused by ischemia. The main objective of this study was to establish a procedure for the efficient production of cardiomyocytes by reprogramming mesenchymal stem cells from adipose tissue. First, lentiviral vectors expressing ...

Abstrad C127 is a nontumorigenic mouse cell line widely used in in vitro transformation assays due to its normal morphological appearance and its very low levels of spontaneous transformation. We now report that C127 cells are resistant to transformation by tyrosine protein kinase oncogenes derived from growth fador receptors such as the retroviral v-fms and the human trk transforming genes. In...

Efficient transfer of the beta-globin gene into primitive hematopoietic progenitors was achieved with consistent and significant expression in the progeny of those cells. Retroviral vectors containing the intact genomic human beta-globin gene and the neomycin (G418)-resistance (neoR) gene were constructed. These gave titers of 10(6) or more neoR colony-forming units/ml when packaged in psi 2 ce...

Gene therapy of human T-lymphocyte disorders, including acquired immunodeficiency syndrome (AIDS), would be greatly facilitated by the development of an in vivo system in which transduced human hematopoietic stem cells can be used to reconstitute the T-lymphoid compartment. Here we use the SCID-hu mouse as a recipient for human CD34+ hematopoietic progenitor cells transduced in vitro with a ret...

An avian leukosis virus-based packaging cell line was constructed from the genome of the Rous-associated virus type 1. The gag, pol, and env genes were separated on two different plasmids; the packaging signal and the 3' long terminal repeat were removed. On a plasmid expressing the gag and pol genes, the env gene was replaced by the hygromycin resistance gene. The phleomycin resistance gene wa...

A procedure is described that directs the self-induced deletion of DNA sequences as they pass through the male germ line of mice. The testes-specific promoter from the angiotensin-converting enzyme gene was used to drive expression of the Cre-recombinase gene. Cre was linked to the selectable marker Neor, and the two genes flanked with loxP elements. This cassette was targeted to the Hoxa3 gene...

a) Water Resources Laboratory, Helsinki University of Technology, 02015 HUT, Finland (emails: [email protected], [email protected], [email protected], [email protected], [email protected]) b) Finnish Environment Institute (SYKE), Mechelininkatu 34a, 00251 Helsinki, Finland (email: [email protected]) c) Environmental Impact Assessment Centre of Finland Ltd (EIA Ltd), Tekniikanti...

nature has always proven that it is able to overcome its problems. however, humanmanipulation has led to environmental degradations. the dryness of a thousand-year urmia lake(a brine water lake in iran) is an example of environmental degradation that happened due tosuccessive droughts and construction of dams on the basin of this lake. this study examinesmethods for the revival of urmia lake by...

According to the water resources and climate change and challenges of Urmia Lake basin, which is the discharge and final destination of North West Rivers, a model was presented. Due to climate change and water resources in river basin such as rainfall, climate change in basin that has direct impact on evaporation over water catchment areas and lake water, this model can be provided. In addition...

The maintenance of T lymphocytes in vivo after adoptive transfer for immunotherapy requires the systemic administration of interleukin-2 (IL-2), but prolonged administration of IL-2 is associated with substantial toxicity. The constitutive production of IL-2 by T cells may be an alternative method to prolong T-cell survival and potentially augment antitumor responses. To study the effects of co...