ANALYSIS OF NON-ESTERIFIED FATTY ACIDS IN HUMAN PLASMA BY CAPILLARY GAS-CHROMATOGRAPHY WITH SPLITLESS INJECTION. The aim of the present work was to test the combination of non-esterified fatty acid (NEFA) isolation using fumed silicon dioxide with capillary gas-chromatography (C-GC) with splitless injection for the analysis of NEFAs in human plasma. Injection volume, solvent re-condensation and...

The physiological response to starvation involves increased muscle proteolysis and adipose tissue lipolysis that supply amino acids and non-esterified fatty acids ('free fatty acids') for gluconeogenesis, oxidation and ketogenesis. In the present issue of Clinical Science, Moller and co-workers show that, in humans, IHL (intrahepatic lipid) content, measured using (1)H-magnetic resonance spectr...

the aim followed in the present study was to investigate the effects of different metabolizable protein (mp) levels on holstein cows, milk yield and composition, and as well on concentration of plasma metabolites in the cow,s early lactation. twenty - four holstein cows(bw 580±23, dim 30±7, parity 2.4±0.5) were randomly assigned to each one of the 4 dietary treatments containing 1) 10.60% (diet...

Background: It was hypothesized that under apparently good management practices in dairy farms, some cows may not be metabolically perfect during the breeding period and this may affect their performance. Aims: This study was conducted to assess probable metabolic drawbacks in mid-lactation dairy cows affecting their performance. Methods: Thirt...

In this study fatty acid contents, because of the importance in human’s life, and their seasonal variations of a dominant bivalve of Haleh Creek were determined for the first time. Fatty acid identification was done by GC-MS (Gas Chromatography–Mass Spectrometry) method. After collecting and dissecting the species from the shells, samples weighed and frozen for further experiments. All the samp...

Lipid composition of the fat body and haemolymph of male milkweed bug, On.cc@eltus fasciatus, was determined. Triglycerides were the predominant lipids of the fat body while diglycerides accounted for the major lipid in the haemolymph. Sterols, sterol esters, and non-esterified fatty acids were present in both fat body and haemoIymph besides triglycerides and diglycerides. Only traces of monogl...

Fatty infiltration of the liver is a rather common lesion, and may be encountered in many different pathological conditions. Notwithstanding the fact that both morphological and chemical aspects are rather uniform, the pathogenesis may be quite different according to the nature of injury. In order to understand the different mechanisms leading to a fatty liver, one has to bear in mind the basic...

We compared UCP3 protein in rat cardiac, glycolytic and oxidative skeletal muscle and examined the effect of high-fat medium chain vs. long chain triacylglycerol feeding on UCP3 content in these tissues. Cardiac muscle displays the lowest basal levels of UCP3 protein. Increasing long chain - but not medium chain - fatty acid supply upregulates UCP3 in all muscles. Since plasma non-esterified fa...