Background The primary goal of any recombinant protein production is to achieve successful gene transfer and expression in a target cell. There are two general categories of delivery vehicles/vectors employed in protein expression protocols. The first category includes the non-viral vectors, ranging from direct injection of DNA to complexing DNA with cationc lipds, polylysine, etc. The second c...

objective: dna vaccines have been widely used to develop immunity against various pathogens including parasites and viruses. the potential of dna vaccine to induce an effective immune response is related to the expression levels of the encoded protein in eukaryotic cells. therefore, optimization of plasmid dna delivery system is a major concern in protein expression in order to make an efficien...

For a variety of reasons, including production limitations, potential unanticipated side effects, and an immunological response upon repeated systemic administration, virus-based vectors are as yet not ideal gene delivery vehicles, justifying further research into alternatives. Unlike viral vectors, non-viral vectors pose minimal health risks, but to meet therapeutic requirements their efficacy...

Gene therapy relies on the delivery of therapeutic genes into patients’ cells. The microdevices used to reach the cells and to transfer the gene payload are called gene vectors. Viral packaging machinery is often utilized to generate the particles transporting the cargo genes. Lentiviruses, a subgroup of retroviruses, are highly suitable for remodeling into gene transfer vectors because they of...

Duchenne muscular dystrophy (BMD) is an inherited X-link disease. The incidence of this muscle-wasting disease is 1:5000 male live births. Mutation in the gene coding for dystrophin is the main cause of BMD. Most cases of this disease succumb to respiratory and cardiac failure in 3rd to 4th decades. The slow progression of BMD and recent achievement of gene therapies make it as an appropriate c...

Gene therapy research is still in trouble owing to a paucity of acceptable vector systems to deliver nucleic acids to patients for therapy. Viral vectors are efficient but may be too dangerous. Synthetic non-viral vectors are inherently safer but are currently not efficient enough to be clinically viable. The solution for gene therapy lies with improved synthetic non-viral vectors systems. This...

background: bovine viral diarrhea virus (bvdv) causes significant economic and health effects in cattle farms. because of the relative inefficiency of bvdv eradication programs, in recent years, numerous strategies such as anti-viral gene therapy has been used extensively to fight it. one of the ways to evaluate the quality of these anti-viral strategies is the study of their efficacy in the sp...