Functional N-methyl-d-aspartic acid (NMDA) receptors are formed from the assembly of NR1 and NR2 subunits. When expressed alone, the major NR1 splice variant and the NR2 subunits are retained in the endoplasmic reticulum (ER), reflecting a quality control mechanism found in many complex multisubunit proteins to ensure that only fully assembled and properly folded complexes reach the cell surfac...

Bacterial recognition of host sialic acid-containing receptors plays an important role in microbial colonization of the human oral cavity. The sialic acid-binding adhesin of Streptococcus gordonii DL1 was previously associated with the hsa gene encoding a 203-kDa protein. The predicted protein sequence consists of an N-terminal nonrepetitive region (NR1), including a signal sequence, a relative...

N-Methyl-D-aspartate (NMDA) receptors play a critical role in both development of the central nervous system and adult neuroplasticity. However, although the NMDA receptor presents a valuable therapeutic target, the relationship between its structure and functional properties has yet to be fully elucidated. To further explore the mechanism of receptor activation, we characterized two gain-of-fu...

N-Methyl-D-aspartate (NMDA) receptors are a member of the ionotropic glutamate receptor family, ligandgated ion channels that mediate the majority of excitatory neurotransmission in the mammalian CNS. They are expressed primarily in the CNS but also in peripheral locations such as pancreatic islet cells, sensory nerve terminals in skin and cardiac ganglia1. Seven subunits of the NMDA receptor h...

Background Abnormal protein expression of N-methyl-D-aspartate (NMDA) receptors essential subunits (NR1, NR2) and of associated post-synaptic density proteins (PSD-95, PSD-93) were observed in schizophrenic patients (postmortem studies) [1,2]. NMDA receptors containing NNR2A/B subunit associate in vivo at synapses with PSD-93 and PSD-95 [3]. In the present study, we have silenced the expression...

We report the phospha-bora-Wittig reaction for direct preparation of phosphaalkenes from aldehydes, ketones, esters, or amides. The transient phosphaborene Mes*P?B–NR2 reacts with carbonyl compounds to form 1,2,3-phosphaboraoxetanes, analogues oxaphosphetane intermediates in classical Wittig reaction. 1,2,3-Phosphaboraoxetanes undergo thermal Lewis acid-promoted cycloreversion, yielding phospha...

A variety of mononuclear alkene complexes, [Ir{ArNC(NR2)NAr}(cod)], and dicarbonyl complexes, [Ir{ArNC(NR2)NAr}(CO)2] (where R = Me or Et; Ar = Ph, 4MeC6H4, 4-MeOC6H4, 2,6-Me2C6H3 or 2,6-Pr2C6H3; and cod = 1,5-cyclooctadiene), were synthesized from the neutral N,N-dialkyl-N′,N′′-diarylguanidines via deprotonation and transmetalation. These complexes were fully characterized by spectroscopic tec...

Agricultural practices such as fertilization considerably influence soil greenhouse gas fluxes. However, the effects of on gases fluxes remain unclear in tea when nitrogen is low. In present study, CO2 and CH4 under various treatments were investigated during a 50-day period. The experiment consisted five treatments: no fertilizer (CK), single (urea, N), oilseed rape cake (R), + (2:1, NR1), (1:...

The antitumor drugs NB-506 and J-107088 are potent topoisomerase I inhibitors with an indolocarbazole structure. To clarify the factors involved in resistance to these drugs, we established two NB-506-resistant mouse fibroblast cell lines (LY/NR1 and LY/NR2), a human colon carcinoma cell line (HCT116/NR1), and a lung cancer cell line (PC13/NR1). These cell lines were highly resistant to NB-506 ...