We have designed and built a data-base system for the storage of nucleic-acid sequences. The system consists of a data base ("the library") and software that manages and provides access to that data base ("the Librarian").

A novel bridged nucleic acid (BNA) analogue, 2 0-O,4 0-C-methyleneoxymethylene bridged nucleic acid (2 0,4 0-BNA), was synthesized and incorporated into oligonucleotides. The 2 0,4 0-BNA modified oligonucleotides showed high binding affinity with an RNA complement and significant enzymatic stability against snake venom phosphodiesterase. 2005 Elsevier Ltd. All rights reserved. 2',4'-BNA/LNA (Re...

The specific hybridization of complementary sequences is an essential property of nucleic acids, enabling diverse biological and biotechnological reactions and functions. However, the specificity of nucleic acid hybridization is compromised for long strands, except near the melting temperature. Here, we analytically derived the thermodynamic properties of a hybridization probe that would enable...

cytotoxicity evaluation of daphne mucronata (thymelaeaceae) extract and one of its active purified components, using seven different cancerous cell lines, revealed the strong antiproliferative activity of the plant. using flow cytometry technique, it was found that treatment of the most responsive cells (k562) with the plant extract or the active component inhibited the progression of cells thr...

The unique properties of fluorine atom on nucleic acid backbone can offer stricking functional and structural features. In order to extend the biological applications L-type acid, we chemically incorporate fluoro-modification into 2′-position L-cytidine, obtain a series fluoro-modified L-DNAs/ L-RNAs. Our melting study indicates that 2′-fluoro-modification does not disrupt thermostabilities wil...