نتایج جستجو برای: nucleic acid sequencing
تعداد نتایج: 867536 فیلتر نتایج به سال:
By Peter C. Iwen, PhD, Associate Director, NPHL For more than 100 years, Robert Koch’s postulate that required in part the cultivation of a pathogen to show a disease/pathogen relationship, was seldom questioned and was considered the basic standard used in clinical diagnostics. Organism identification to taxon (species, genus) was subsequently accomplished by studying phenotypic characteristic...
Scoring matrices for nucleic acid sequence comparison that are based on models appropriate to the analysis of molecular sequencing errors or biological mutation processes are presented. In mammalian genomes, transition mutations occur significantly more frequently than transversions, and the optimal scoring of sequence alignments based on this substitution model differs from that derived assumi...
Low-cost DNA sequencing technologies have expanded the role for direct nucleic acid sequencing in the analysis of genomes, transcriptomes, and the metagenomes of whole ecosystems. Human and machine comprehension of such large datasets can be simplified via synthesis of sequence fragments into long, contiguous blocks of sequence (contigs), but most of the progress in the field of assembly has fo...
Abstract Nucleic acid aptamers are generated by an in vitro molecular evolution method known as systematic of ligands exponential enrichment (SELEX). Various candidates limited actual sequencing data from experiment. Here we developed RaptGen, which is a variational autoencoder for silico aptamer generation. RaptGen exploits profile hidden Markov model decoder to represent motif sequences effec...
The use of endoscopic ultrasonography has allowed for improved detection and pathologic analysis of fine needle aspirate material for pancreatic lesion diagnosis. The molecular analysis of KRAS has further improved the clinical sensitivity of preoperative analysis. For this reason, the use of highly analytical sensitive and specific molecular tests in the analysis of material from fine needle a...
Structural features of nucleic acids have become an integral part of current biomedical research. Highly selective and readily performed methods with little toxicity that target guanosines in non-duplex nucleic acids are needed, which led us to search for an effective agent for guanosine sequencing. Treatment of DNA or RNA with potassium tungstate and hydrogen peroxide produced damaged guanosin...
Curation and storage of formalin-fixed, paraffin-embedded (FFPE) samples are standard procedures in hospital pathology laboratories around the world. Many thousands of such samples exist and could be used for next generation sequencing analysis. Retrospective analyses of such samples are important for identifying molecular correlates of carcinogenesis, treatment history and disease outcomes. Tw...
Since the early 1970s, the use of nucleic acid sequences for specific diagnostic applications has followed a somewhat linear pattern of development. Early methods for restriction enzyme digestion, as well as reverse transcription, were followed in the late 1970s by Southern, northern and dot blotting, as well as DNA sequencing. In 1985, the description of PCR and the routine laboratory manipula...
OVERVIEW: After sequencing the entire human genome, researchers are now turning their attention to clinical gene testing and analysis to explain gene function and differences among individuals. To research and to test genetic material, efficient and simplified techniques to extract nucleic acid from blood samples are essential. Hitachi has developed a new aspiratedischarge method, which incorpo...
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