BACKGROUND Application of assisted reproductive technologies in buffaloes is limited to some extent by farmers' inability to detect oestrus because of its poor expression. The present study aimed at investigating reliability of a milk progesterone enzyme-linked immunosorbent assay (ELISA) to assess the ovarian cyclicity during post partum, oestrus and post-breeding periods in water buffaloes. ...

A 2-sample regime was used to measure whole milk progesterone concentration on the day of oestrus and insemination (Day 0) and 6 days later (Day 6) in a sample of 50 primiparous and 100 multiparous suckling beef cows. Exposure to teaser bulls and observation by cattlemen identified the occurrence of oestrus. Three sets of criteria used to define ovulatory oestrus were compared: a) milk progeste...

The objective was to quantify and compare cow cervical morphology on oestrus vs. non-oestrus daysusing a new farm technology. The cervical tissues were videotaped using a cervixscope involving newcamera equipment in four Holstein cows on multiple oestrus and non-oestrus days to score tissue morphology as altered by oestrus. The non-oestrous days were in diestrus phases. The videotaped records w...

In modern high-yielding dairy herds fertility is of major economic importance. In order to gain maximum profit, calving intervals should not exceed 365 days. The achievement of a 365-day calving interval requires an early resumption of ovarian activity, an excellent oestrus detection, and a high first-service conception rate. Especially the inability to detect oestrus and to mate the cows by 60...

For herds using AI heat detection rate and calving rate are the two major determinants of compactness of calving, of the proportion of cows that fail to conceive in a defined breeding season. Numerous factors affect the expression of heat including, housing arrangement, floor surface, feet and leg problems and status of herd mates. The number of mounts a cow receives increases with the number o...

In October, 1964, beef cows were divided into three groups. One group was run with bulls for four months (group 1); another group was kept free of bulls and was observed daily for six weeks and cows detected in oestrus were inseminated using chilled semen (group 2); and the third group was given progestagens to synchronise ovarian cycles and the cows were inseminated after treatment and again a...

Fladioimmunoassay technique was used to determine the concentrations of progesterone in serum, fore-milk and saliva of 40 Nili-Ravi buffaloes during pro-oestrus, oestrus, met-oestrus and di-oestrus. Progesterone concentrations during oestrus were uniformly lower in all experimental body fluids; the highest concentrations were found during the mid luteal phase. Although the hormonal trend was si...

Numerous studies have shown that it is possible to manipulate follicular and luteal dynamics, thereby eliminating the need for oestrus detection in embryo transfer (ET) programmes. Fixed-time ET (FTET) protocols are based on the use of gonadotrophin-releasing hormone (GnRH) and prostaglandin (PG) F or progesterone/progestogen (P4)-releasing devices and oestradiol. The FTET protocols increases t...

Difficulty in observing oestrus is a problem for many dairy farmers performing AI. Finding ways to synchronize oestrous cycles or strengthen display of oestrus without hormonal treatments would be of great interest because many consumers object to the use of exogenous hormones on healthy animals. Modification of reproductive cycles through chemical communication has been reported in several spe...

The present study was conducted to see the effect of non-specific genital infections and ovulatory disturbances on clinical signs of oestrusnamely oestrus duration, tonicity of uterus, relaxation of cervix and persistence of follicle. The mean values of oestrus duration, tonicity of uterus and relaxation of cervix varied significantly (P