Duckweeds (Lemna spp.) are small, free floating aquatic plants that flourish on stagnant or slow moving water surfaces throughout the world. Members of the genus are among the smallest flowering plants, providing food for fish and fowl, but their aggressive growth and invasive tendencies make them formidable aquatic weeds, which when uncontrolled can result in oxygen depletion, fish kills, and ...

Pasteurella multocida serotype A:3 is a Gram-negative bacterial pathogen, one of the causative agents of shipping fever of cattle. In this study, outer membrane protein H (ompH) and Pasteurella lipoprotein E (plpE) genes were cloned and plpEC-ompH fusion was constructed and expressed in Escherichia coli. Recombinant PlpE, OmpH and PlpEC-OmpH fusion proteins were purified and formulated with oil...

Received: Revised: Accepted: Online available: October 10, 2015 December 31, 2015 January 02, 2016 January 13, 2016 Hemorrhagic septicemia is an important disease of cattle and buffaloes in Asia, particularly the South and Southeast Asia. Since outbreaks lead to numerous deaths and severe economic loss, control by vaccination is extremely important. However, the current use of broth vaccine for...

Trichinellosis, a re-emerging zoonosis in several countries and pig, is the main species responsible for its transmission to human. Vaccination of swine could be an alternative to prevent the risk of human contamination. In order to develop an efficient and safe inactivate vaccine, the choice of the adjuvant is an important issue. The aim of this study was to develop and select potent and safe ...

Infectious bovine rhinotracheitis (IBR) virus was grown in Madin Darby bovine kidney (MDBK) cell line using a roller culture system for its large-scale production. Optimum multiplicity of infection (MOI) of 1:750 was found to give consistent virus yield. To determine the appropriate payload, three batches of antigen with virus titres ranging from 10(8.37) to 10(6.37) TCID50 per ml were used to ...

BACKGROUND Adjuvants can improve pesticide application efficiency and effectiveness. However, quantifications of the adjuvant-amended pesticide droplet actions on foliage, which could affect application efficiencies, are largely unknown. RESULTS Droplet evaporation rates and spread on waxy or hairy leaves varied greatly with the adjuvant types tested. On waxy leaves, the wetted areas of dropl...

Abstract Background and Aims: Vaccination of poultry has a major impact on the prevention and control of avian influenza viruses. Nanobiotechnology techniques provide a new approach for improvement of influenza vaccine efficacy. In this study, efficacy of an inactivated nano-adjuvant vaccine developed based on an endemic H9N2 virus was evaluated in SPF chickens. Materials and Methods: I...

The effect of polyadenylic acid: polyuridylic acid complex (poly A: U) on immunization was investigated in mice vaccinated by killed Brucella melitensis cells suspended in incomplete adjuvant or in saline. Addition of 300 ,ug of poly A:U to vaccines rendered 3 X 108 B. melitensis cells in saline as immunogenic as 3 X 10"1 cells in oil adjuvant against a severe B. abortus challenge. In the mouse...

Water-in-oil emulsions are commonly used to provide persistent release of antigens necessary for sustained stimulation of the immune system. Optimal response of an animal's immune system is dependent upon repeated, maintained exposure to the antigen. The sustained release of antigen, which delays catab-olism, is thought to be one of the major methods by which adjuvants enhance the immune respon...