We describe 12 diagnostic single nucleotide polymorphism (SNP) assays for use in species identification among rainbow and cutthroat trout: five of these loci have alleles unique to rainbow trout (Oncorhynchus mykiss), three unique to westslope cutthroat trout (O. clarkii lewisi) and four unique to Yellowstone cutthroat trout (O. clarkii bouvieri). These diagnostic assays were identified using a...

Spatial and temporal fluctuations in productivity and abundance confound assessments of captive propagation programs aimed at recovery of Threatened and Endangered populations. We conducted a 17 year before-after-control-impact experiment to determine the effects of a captive rearing program for anadromous steelhead trout (Oncorhynchus mykiss) on a key indicator of natural spawner abundance (na...

Non-indigenous species may be the most severe environmental threat the world now faces. Fishes, in particular, have been intentionally introduced worldwide and have commonly caused the local extinction of native fish. Despite their importance, the impact of introduced fishes on threatened populations of Pacific salmon has never been systemically examined. Here, we take advantage of several uniq...

Visible implant alpha (VI alpha) tag-induced changes in mortality and condition, as well as tag retention and readability, were examined during a 4-week period for juveniles of three fish species: tiger muskellunge Esox masquinongy × Esox lucius (91 ± 7 mm total length, L(T), mean ± s.d.), Snake River cutthroat trout Oncorhynchus clarki behnkei (84 ± 8 mm) and rainbow trout Oncorhynchus mykiss ...

Tissue-specific expression of three L-rhamnose-binding lectins, named STL1, STL2, and STL3, in the steelhead trout (Oncorhynchus mykiss) was investigated. STL2 and STL3 mRNAs were restricted in the oocytes. In contrast, STL1 mRNA was detected only in the liver. The transcription of STL2 and STL3 started in previtellogenic oocytes. These results showed distinct expression profiles of rhamnose-bi...

Rainbow trout (Oncorhynchus mykiss) head kidney phagocytes precultured with a synthetic cytidine-phosphate-guanosine (CpG) oligodeoxynucleotide (ODN) displayed significantly higher phagocytic activity against Vibrio ordalii than phagocytes precultured with non-CpG ODN. However, head kidney phagocytes precultured with CpG ODN did not show enhanced phagocytic activity against polystyrene particles.

The ultra-fast quantitative real-time polymerase chain reaction (qPCR) assay was developed and validated to differentiate the morphologically similar ones, Oncorhynchus keta mykiss. Species-specific primers were designed for COI genes of mtDNA. species-specific O. mykiss selectively amplified by DNA, respectively. sensitivity 1 ng/μL. Quantitative testing showed that results met ‘Guidelines on ...

TWO STRATEGIES HAVE BEEN PROPOSED TO AVOID NEGATIVE GENETIC EFFECTS OF ARTIFICIALLY PROPAGATED INDIVIDUALS ON WILD POPULATIONS: (i) integration of wild and captive populations to minimize domestication selection and (ii) segregation of released individuals from the wild population to minimize interbreeding. We tested the efficacy of the strategy of segregation by divergent life history in a ste...