Organophosphorus (OP) pesticides can be rapidly detected by integrating organophosphorus hydrolase with an optical leaky waveguide biosensor. This enzyme catalyses the hydrolysis of a wide range of organophosphorus compounds causing an increase in the pH. Thus, the direct detection of OP is possible by monitoring of the pH changes associated with the enzyme's activity. This article describes th...

It is shown from the kinetics of inhibition of cholinesterase by N-methylcarbamates and organo-phosphates that the LD50 dose is likely to be a much greater multiple of the dose causing signs of poisoning in 50%-of the animals (the ED50) for the carbamates than for the organophosphates. The expected difference was demonstrated by a comparison of the LD50s and ED50s, intravenous and intramuscular...

An overview of general strategy, standard procedures, and critical points, which may be found during carrying out an OPCW Proficiency Test concerning detection and identification of scheduled compounds relevant to Chemical Weapon Convention, has been presented. The observations have been illustrated following the case of the Eight OPCW Designated Laboratories Proficiency Test, which was perform...

It has been shown that several hydrolytic enzymes with different specificities undergo similar reactions with diisopropyl phosphorofluoridate (1) and other related toxic organophosphorus compounds (2). These conclusions were based on kinetic studies, particularly the influence of pH on the rates of reaction, and on inhibition characteristics. Reactions of this type have provided a unique method...

1 Associate Professor, Department of Biochemistry, R.C.S.M. Govt. Medical College, Kolhapur 2 Technician, Department of Biochemistry, Govt. Medical College, Miraj 3 Ph D student, Department of Biochemistry, Govt. Medical College, Miraj Corresponding Author Dr Karan Ningappa Pujari Associate professor, Department of Biochemistry, R.C.S.M. Govt. Medical College, Kolhapur (Maharashtra, India). Ema...

As a consequence of organophosphorus (OP) toxins posing a threat to human life globally, organophosphorus hydrolase (OPH) has become the enzyme of choice to detoxify such compounds. Organophosphorus hydrolase was covalently immobilized onto a quartz substrate for utilization in paraoxon detection. The substrate was cleaned and modified prior to chemical attachment. Each modification step was mo...