BACKGROUND Diagnosing canine immune-mediated haemolytic anaemia (IMHA) is often challenging because all currently available tests have their limitations. Dogs with IMHA often have an increased erythrocyte osmotic fragility (OF), a characteristic that is sometimes used in the diagnosis of IMHA. Since the classic osmotic fragility test (COFT) is time-consuming and requires specialized equipment, ...

The Erythrocyte Osmotic Fragility (EOF) in case of anaemic cattle mostly affected with haemolytic anaemia were evaluated in this study. As haemolytic anaemia is a consistent finding in different blood protozoan diseases like theileriosis and trypanosomiasis, determination of EOF in these cases shows the level of osmotic stress in the affected animals. The present study was also aimed to determi...

The survival of erythrocytes (RBC) is shortened in uremia, and it has been shown that calcium influx into RBC evoked crenation and increased their rigidity. The high blood levels of parathyroid hormone (PTH) may augment entry of calcium into RBC and hence affect their integrity. We examined the effect of PTH on osmotic fragility of human RBC and investigated the mechanisms through which PTH int...

  Mild anaemia among patients with hyperthyroidism, reduction in intracranial zinc content of erythrocytes and anti- haemolysis of whole blood of patients with Graves, disease under laboratory Condition are the facts which indicate that although erythrocytes are devoid of receptors for thyroid hormones, they are affected by the excess of these hormones. In order to find out the reasons for suc...

To investigate the effects of two widely used anticoagulants and storage on the in vitro osmotic fragility of erythrocytes, haematocrit (Hct) and plasma osmolality of ostriches, blood was collected by venipuncture from 8 birds (90-110 kg) into vacutainer tubes containing either EDTA (k3) or lithium heparin. The osmotic fragility of the erythrocytes was determined by measuring the release of hae...

The phosphatidylcholine specific transfer protein (PCTP) from bovine liver was used to retailor the molecular species composition of phosphatidylcholine (PC) in the membrane of normal (AA) and sickleable (SS) human erythrocytes. Changes in molecular species composition of PC altered morphology as well as cellular deformability and stability as measured with ektacytometry. In normal cells. repla...

A computer simulation of osmotic processes has been developed. Several experimental setups are graphically simulated and may be freely selected by the user. The simulation analyzes the basic factors which determine the osmotic pressure and volume flow of a solution (e.g. concentration, temperature, activity coefficient, Staverman's coefficient and external pressure). The program was written in ...

Three-dimensional (3D) ac electro-osmotic (ACEO) pumps have recently been developed that are much faster and more robust than previous planar designs. The basic idea is to create a "fluid conveyor belt" by placing opposing ACEO slip velocities at different heights. Current designs involve electrodes with electroplated steps, whose heights have been optimized in simulations and experiments. Here...

Mice of strain DBA/2J were found to produce red cells considerably more resistant to osmotic lysis than cells from C57BL/6J or the F1 hybrid between the two strains. Such strain-specific differences in osmotic fragility could be the result of genetically determined humoral or other systemic differences that indirectly influence red cell properties. Alternatively, this phenotypic variation might...

The strategy for screening of thalassemia and Hb E by a combination of osmotic fragility (OF) test and dichlorophenol indophenol precipitation (DCIP) test was validated with 436 unrelated Thai subjects. Hemoglobin (Hb) typing, Hb A2 quantitation, PCR and DNA sequence analysis were used as confirmatory methods for diagnosis of thalassemia and hemoglobinopathy. The sensitivity and specificity of ...