background: ovarian tissue cryopreservation is an alternative strategy to preserve the fertility of women predicted to undergo premature ovarian failure. this study was designed to evaluate the expression of folliculogenesis-related genes, including factor in the germline alpha (figla), growth differentiation factor-9 (gdf-9), follicle-stimulating hormone receptor (fshr), and kit ligand after v...

background: many attempts have done to improve cryopreservation of mammalian ovaries using simple, economical and efficient technique “vitrification”. objective: the aim of the present study was to compare the mouse ovaries cryopreservation by direct cover vitrification (dcv) using different concentrations of ethylene glycol (eg) with conventional vitrification methods (cv). materials and metho...

STUDY QUESTION Is ovarian tissue cryopreservation using vitrification followed by in vitro activation (IVA) of dormant follicles a potential approach for infertility treatment of patients with primary ovarian insufficiency (POI)? SUMMARY ANSWER Our vitrification approach followed by IVA treatment is a potential infertility therapy for POI patients whose ovaries contain residual follicles. W...

OBJECTIVE To evaluate the safety and risk of cryopreservation in female fertility preservation. DATA SOURCES The data analyzed in this review were the English articles from 1980 to 2013 from journal databases, primarily PubMed and Google scholar. The criteria used in the literature search show as following: (1) human; embryo; cryopreservation/freezing/vitrification, (2) human; oocyte/immature...

PURPOSE To investigate the effect of antifreeze protein (AFP) supplementation on ovarian vitrification and transplantation. MATERIALS AND METHODS In this experimental study, we researched a total of 182 ovaries from 4-week-old ICR mice. The equilibration solution included 20% ethylene glycol (EG), and the vitrification solution included 40% EG, 18% Ficoll, and 0.3 M sucrose. Intact ovaries we...

Background This research was conducted to assess preantral follicle development in fibrin alginate matrix following ovarian tissue vitrification MaterialsAndMethods Ovaries of 13-day-old NMRI female mice were removed and placed in control and vitrification groups. Vitrification group ovaries were transferred in media containing ethylene glycol, dimethyl sulphoxide, and sucrose then were plunged...

Background The high effectiveness of vitrification, as a cryopreservation protocol, for human oocytes and embryos is shown, whereas data on human ovarian tissue are limited. This study aimed to assessment of follicular growth, ultrastructure, and apoptosis incidence in human ovarian tissue following vitrification/warming and after culture in the presence of LIF. MaterialsAndMethods Ovarian cort...

Cryopreservation of avian ova and embryos is challenging because of the yolky structure of the egg. As an alternative, with the development of effective cryopreservation protocols, ovarian tissue cryopreservation could be used for cryobanking for birds. Pieces of ovarian tissue of week-old Japanese quail (Coturnix japonica) were frozen at 0.5 degrees C/min in a programmable freezer or vitrified...

Background: Cryopreservation of ovary for preserving germ cell line is going to be an important issue. However, the physical and chemical stresses during cryopreservation are the most important factors that may influence the post freezing tissue viability and quality. Vitrification is a new approach to bypass the impacts of both stresses for gametes and embryos and it is also suggested for ovar...