نتایج جستجو برای: oxalate oxidase
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بیماری بلایت خوشه گندم یک بیماری ویرانگر در بسیاری از مناطق جهان و ایران به شمار می رود. این بیماری سبب خسارت کمی، کیفی و آلودگی محصول به زهرابه های قارچی می گردد. عمده ترین عامل قارچی بیماری بلایت خوشه گونه fusarium graminearum schwabe با فرم جنسی gibberella zeae می باشد. تحقیقات وسیعی برای مدیریت این بیماری از طریق بکارگیری ارقام مقاوم و متحمل، استفاده از قارچ کش ها و ارائه سیستم های پیش آگاه...
In an attempt to decrease ascorbate interference on the ion-chromatographic determination of urinary oxalate, we compared the effectiveness of four different methods for ascorbate elimination by analyzing a representative urine pool supplemented with successive ascorbate additions. Two of the methods--treatment with ferric ions or boric acid--have been described elsewhere; treatments with nitri...
Oxalate in urine was measured by grain Sorghum leaf oxalate oxidase conjugated to alkyl amine glass beads affixed in a beaker. The minimum detection limit was 0.05 mM/L in urine. Recovery of added oxalate in urine was 80.5% and within and between assay, coefficients of variation (CV) were <4% and <5.5%, respectively. Urinary oxalate values obtained by the present method showed a good correlatio...
Oxalate oxidase (E.C. 1.2.3.4) catalyses the conversion of oxalate and dioxygen to carbon dioxide and hydrogen peroxide (for reviews see [ 1-31). Oxalate oxidase activity has been detected in a number of organisms but the best biochemically characterised enzymes are from barley and wheat. The plant enzyme is isolated as a glycosylated homopentamer which is remarkably resistant to heat, SDS and ...
Effective pathogenesis by the fungus Sclerotinia sclerotiorum requires the secretion of oxalic acid. Studies were conducted to determine whether oxalate aids pathogen compatibility by modulating the oxidative burst of the host plant. Inoculation of tobacco leaves with an oxalate-deficient nonpathogenic mutant of S. sclerotiorum induced measurable oxidant biosynthesis, but inoculation with an ox...
This new reagent kit for the quantitative measurement of oxalate in urine is a modification of an earlier Sigma oxalate assay procedure (procedure no. 590), a coupled enzyme assay involving oxalate oxidase and horseradish peroxidase. The new analytical procedure includes methods for processing urine specimens to eliminate interference with oxalate color development at 590 nm by ascorbic acid, d...
Primary hyperoxaluria type 1 (PH1) is an autosomal recessive, metabolic disorder caused by mutations of alanine-glyoxylate aminotransferase (AGT), a key hepatic enzyme in the detoxification of glyoxylate arising from multiple normal metabolic pathways to glycine. Accumulation of glyoxylate, a precursor of oxalate, leads to the overproduction of oxalate in the liver, which accumulates to high le...
For this direct colorimetry of urinary oxalate, commercially available oxalate oxidase (EC 1.2.3.4) is used. The urine is first diluted, to diminish the effect of interfering substances. Analytical recovery of oxalate from urines with five different oxalate concentrations (0.4 to 2.0 mmol/L) ranged from 92 to 109% (mean 99%). The within-day and between-day precision (CV) of the method for a wid...
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